International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:7, July, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 3987-3993

Improved Detached Leaf Assay for Rapid Screening against Late Leaf Spot in Groundnut
Dnyaneshwar B. Deshmukh1, Ashwini M. Teggi2, Hari Kishan Sudini2, Sunil Chaudhari2, Balram Marathi1, Ch. V. Durga Rani2 and Murali T. Variath2*
1Institute of Biotechnology, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad- 500 030
2Groundnut Breeding, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru- 502 324
*Corresponding author

The use of resistant cultivars is the most economical and effective method to control late leaf spot (Phaeoisariopsis personata) in groundnut. In the process of breeding for host plant resistance, the plant material is evaluated in the hot spot location under field conditions. But this process of evaluating the whole crop cycle demands lot of labour and time, the disease expression fluctuates with environmental conditions. Here, we present the improved detached leaf assay method for screening groundnut lines under controlled conditions. Three genotypes of groundnut viz., GPBD 4, ICGV 13193 and TMV 2 were screened against late leaf spot. Based on visual observation of incubation period, percentage of leaf area damaged, lesion diameter, latent period and defoliation percentage were recorded. The varieties GPBD 4 and TMV 2 consistently showed lowest and highest disease intensities, respectively. While the introgression line ICGV 13193 showed moderate resistance. This methodology may allow the detection of sources of resistance that specifically involve one or more components of resistance for defence mechanisms against LLS. When combined with field assessment, this methodology should also enable to quantitatively assess the relative role of each components of resistance to LLS. Therefore, detached leaf assay can be used to screen groundnut genotypes against late leaf spot.

Keywords: Arachis, Late leaf spot, Detached leaf assay, Latent period, Per cent leaf area damaged, Host plant resistance

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How to cite this article:

Dnyaneshwar B. Deshmukh, Ashwini M. Teggi, Hari Kishan Sudini, Sunil Chaudhari, Balram Marathi, Ch. V. Durga Rani and Murali T. Variath. 2018. Improved Detached Leaf Assay for Rapid Screening against Late Leaf Spot in Groundnut.Int.J.Curr.Microbiol.App.Sci. 7(7): 3987-3993. doi:
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