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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 3808-3816
DOI: https://doi.org/10.20546/ijcmas.2018.707.442


Quantitative and Morphological Evaluation of the Effect of Platelet Rich Plasma on Collagen Fibers in Experimentally Induced Skeletal Muscles Injury in Adult Male Albino Rats
Shimaa M. Badr*, Reda H Elbakary, Essam M. Laag, Naglaa I. Sarhan and Nafisa A. Elbakary
Histology Department, Faculty of Medicine, Tanta University, Egypt
*Corresponding author
Abstract:

Muscle injuries present frequently in sports medicine. Healing with conventional therapy is often inadequate, leading to incomplete functional recovery due to fibrosis. Generating substantial interest in the potential for emerging technologies such as platelet-rich plasma (PRP) to enhance soft-tissue healing and decrease time of recovery became mandatory. The aim of this work was to evaluate the role of PRP in promoting healing of experimentally induced skeletal muscle injury in adult male albino rat model. 48 adult male albino rats were used and were divided into three groups. Group I was served as control for obtaining muscle specimens from their gastrocnemius muscle and PRP. Group II was exposed to bilateral gastrocnemius muscles injury and was left without treatment. Group III were subjected to bilateral gastrocnemius injury and were immediately treated with PRP intramuscularly. Muscle specimens were excised after 1, 7 and 21 days from the onset of injury to be processed for light microscopic study after staining with Mallory trichrome stain. Assessments of the mean area percentage of collagen fibers were also statistically analyzed. Treatment with PRP resulted in enhanced regeneration of skeletal muscle injury without fibrosis. The PRP treated group demonstrated absence of fibrosis on days 14 and 21 as compared to their associates in non-treated group. Local injection of PRP into the injured gastrocnemius muscle resulted in enhanced muscle regeneration without fibrosis.


Keywords: Platelet rich plasma, Muscle injury, Fibrosis, Mallory trichrome
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How to cite this article:

Shimaa M. Badr, Reda H Elbakary, Essam M. Laag, Naglaa I. Sarhan and Nafisa A. Elbakary. 2018. Quantitative and Morphological Evaluation of the Effect of Platelet Rich Plasma on Collagen Fibers in Experimentally Induced Skeletal Muscles Injury in Adult Male Albino Rats.Int.J.Curr.Microbiol.App.Sci. 7(7): 3808-3816. doi: https://doi.org/10.20546/ijcmas.2018.707.442