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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 3718-3727
DOI: https://doi.org/10.20546/ijcmas.2018.707.430


Antibacterial Activity and Phytochemical Analysis of Methanolic and Acetonic Extracts from Moringa oleifera, Vitex negundo and Rosa indica
Kruti Dave1, Hardik Shah2 and Kirankumar G. Patel3*
1Department of Biotechnology,
2Department of Microbiology, Mehsana Urban Institute of Sciences, Ganpat University, Gujarat, India
3Department of Biological Sciences, P. D. Patel Institute of Applied Sciences, CHARUSAT, Gujarat, India
*Corresponding author
Abstract:

Throughout the ages, plants have been a valuable resource of natural products for human health. All parts of the plant, from root to fruit, consisting of a multitude of secondary metabolites which impart an unprecedented variety of medicinal uses to the plant. Studies have shown the presence of different phytochemical constituents in botanical sample responsible for the antimicrobial activity. These antimicrobial agents should be beneficial to host cells and toxic to pathogenic microbes. Some medicinal plants and spices have been reported to exhibit antibacterial activity. Hence, the antibacterial activity was examined from the leaf of Moringa oleifera and Vitex negundo and petals of Rosa indica through agar disc diffusion method. Each sample was collected and its crude extract was obtained by using methanol and acetone as the extraction solvent. These extract were tested against two Gram positive (Bacillus cereus and Staphylococcus aureus) and one Gram negative bacteria (Salmonella typhi). The methanolic and acetonic extract of each M.oleifera, V.negundo and R. indica showed antibacterial activity against Bacillus cereus, Staphylococcus aureus and Salmonella typhi. Bio-chemical test for the presence of phytochemicals have shown positive result for tannin, flavonoid and alkaloid. These phytochemicals have ability to fight against microorganisms or inhibit the growth of microorganisms. This approach will be an advanced step in the discovery of some herbal drugs. These plant extracts which were proven to be potentially effective can be used as a natural alternative to the chemical preservatives frequently used in food preparations. It could be an ideal way to avoid health hazards that may occur due to chemical antimicrobial agents.


Keywords: Antibacterial activity, Phytochemical analysis, Acetonic extracts, Moringa oleifera, Vitex negundo and Rosa indica
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How to cite this article:

Kruti Dave, Hardik Shah and Kirankumar G. Patel. 2018. Antibacterial Activity and Phytochemical Analysis of Methanolic and Acetonic Extracts from Moringa oleifera, Vitex negundo and Rosa indica.Int.J.Curr.Microbiol.App.Sci. 7(7): 3718-3727. doi: https://doi.org/10.20546/ijcmas.2018.707.430