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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 3702-3713
DOI: https://doi.org/10.20546/ijcmas.2018.707.428


Region based Diversity Studies of Saccharomyces cerevisiae and Lentinula edodes
Keshani1, Shivani Sharma1*, S.S. Kanwar2, P.K. Khanna1 and Shammi Kapoor1
1Department of Microbiology, College of Basic Sciences and Humanities, Punjab Agricultural University, Ludhiana 141004, Punjab, India
2Department of Microbiology, College of Basic Sciences, Himachal Pradesh Agricultural University, Palampur 176062, HP, India
*Corresponding author
Abstract:

Microbial diversity is the main key point during the studies of microbial ecology. The diversity of edible fungal species in an area affects the nutritional behavior of that community. Hence, the present study has been designed for assessing the diversity of two very important fungal genera involved in eating habits of the Indians i.e. Saccharomyces cerevisiae and Lentinus edodes using different molecular as well as protein markers. The results revealed the divergent nature of these two genera on the basis of their geographical origins. PCR-RFLP analysis of S. cerevisiae strains from Northern India clustered them into two groups one with four strains from different locations and other with fourteen strains, most of which from the same geographical location i.e. Lahaul and Spiti. Two indigenous strains of L. edodes from Tamil Nadu Agricultural University, Coimbatore (LeC and LeI) showed different characteristics as compared to other strains as seen by protein profiling using SDS-PAGE and isozymes. This study gives an indication of these techniques to be a powerful tool to discriminate these fungal species at intraspecies level for their diversity analysis.


Keywords: Saccharomyces cerevisiae, Lentinula edodes, Diversity, PCR-RFLP and SDS-PAGE
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How to cite this article:

Saccharomyces cerevisiae, Lentinula edodes, Diversity, PCR-RFLP and SDS-PAGEInt.J.Curr.Microbiol.App.Sci. 7(7): 3702-3713. doi: https://doi.org/10.20546/ijcmas.2018.707.428