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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 3590-3603
DOI: https://doi.org/10.20546/ijcmas.2018.707.418


In Vitro Life Cycle, Micromorphological Studies and Reproductive Biology of an Anti-diabetic Fern
M. Gayathiri*, I. Ramya Roselin, S. Sujatha and S. Catharin Sara
PG and Research Department of Botany, Holy Cross College (Autonomous), Tiruchirappalli- 620 002, Tamil Nadu, India
*Corresponding author
Abstract:

Adiantum caudatum Linn. is an important medicinal fern belonging to the family Adiantaceace, coming under Pteridophytes. It is used traditionally in folklore as ethno medicine to treat various diseases like cough, cold, throat infection, skin disease and diabetes. The in vitro life cycle of A.caudatum L. starting from spore germination, gametophyte formation, followed by micro morphological study and reproductionwas thoroughly studied in knop’s (Kn) and Knudson’s medium (Kc).Vittaria type of germination and Drynaria type of gametophytes evidenced on two media studied during the culture period. Presexual gametophyte, sex organ formation, fertilization, embryo development and sprouting of young sporeling were successful in Kc medium under in-vitro conditions. Experimental trials for spore germination percentage, gametophytic growth area and micromorphological differences between Kc and Kn medium studied were observed and interpreted. Bisexual potential, selfing potential and genetic load revealing reproductive potential was found out for the experimental plant studied and discussed. The optimum repeatable protocol for the micropropagation of this lithophytic medicinal fern was attained after 150 days of culture.


Keywords: Adiantum caudatum Linn., In vitro culture, Spore culture protocol, Micromorphology studies, Reproductive potential
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How to cite this article:

Gayathiri, M., I. Ramya Roselin, S. Sujatha and Catharin Sara, S. 2018. In Vitro Life Cycle, Micromorphological Studies and Reproductive Biology of an Anti-diabetic Fern.Int.J.Curr.Microbiol.App.Sci. 7(7): 3590-3603. doi: https://doi.org/10.20546/ijcmas.2018.707.418