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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 3460-3466
DOI: https://doi.org/10.20546/ijcmas.2018.707.401


Evaluation of Bleach Sedimentation Technique in Detection of Mycobacterium tuberculosis by LED Microscopy
Suneet K. Yadav1, R.Sujatha2, D.N. Singh3 and Deepak Sameer4
Department of Microbiology, Rama Medical College Hospital and Research Centre, Mandana, Kanpur, India
*Corresponding author
Abstract:

The bacteriological diagnosis of tuberculosis (TB) is largely dependent on the Ziehl-Neelsen (ZN) microscopy. This method has a low sensitivity, which can be improved by the concentration of the specimen with sodium hypochlorite or Bleach (NaOCl), followed by sedimentation after doing LED. This study was conducted to evaluate the efficiency of bleach sedimentation technique in detection of Mycobacterium tuberculosis by LED microscopy. A Total of 100 sputum samples from the patients who visited RNTCP of Rama Medical College Hospital and Research Centre between April 2015 and March 2016. Morning samples were collected from each patient, direct smears were prepared and they were stained with the ZN Stain and aura LED technique and the remaining samples were concentrated by using 3.5% NaOCl, followed by sedimentation and staining with ZN stain and aura LED stain. The improvement in the sensitivity following the bleach method was studied. Out of 100 sputum samples, 50 smear positive samples by ZN showed 100% positivity but increase the grading of positive result after the bleach sedimentation technique in both LED and ZN microscopy while 50 AFB negative samples shows 12% positivity rate was ZN for bleach and 26% shows positivity rate was using aura LED for bleach sedimentation technique. Whereas the overall positivity rate was 56% shows by ZN for bleach and 63% shows positivity rate was using aura LED for bleach sedimentation technique. A statistically significant (p < 0.0001) increase in the positivity with the use of the bleach method was detected as compared to that with the use of the direct ZN staining and aura LED Method. After bleach for LED is sensitivity, specificity, positive predictive value (PPV), and the negative predictive value (NPV) were 100%, 89.3%, 90.3%and 100% respectively with a 95% confidence interval, with the use of the 3.5% NaOCl method. In the present study, the Bleach sedimentation method increases the positivity in aura LED microscopy when it was compared with the ZN microscopy The bleach method has advantages over the direct method, as it is simple and as it does not require any additional expertise beyond that which is required for the conventional direct smear microscopy. The materials and the reagents are also affordable and they are available locally.


Keywords: Tuberculosis, Acid fast bacilli, Ziehl-Neelsen method, Aura-mine method, Light emitting diode, Sodium hypochlorite
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How to cite this article:

Suneet K. Yadav, R. Sujatha, D.N. Singh and Deepak Sameer. 2018. Evaluation of Bleach Sedimentation Technique in Detection of Mycobacterium tuberculosis by LED Microscopy.Int.J.Curr.Microbiol.App.Sci. 7(7): 3460-3466. doi: https://doi.org/10.20546/ijcmas.2018.707.401