National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus (MRSA) has been considered to be a major cause of healthcare infections worldwide and poses a major threat to public health. It is also one of the main etiological agents which is responsible for clinical and subclinical mastitis in dairy herds. This study was designed to investigate the occurrence of S. aureus and Methicillin resistant Staphylococcus aureus (MRSA) from bovine raw milk by Polymerase chain reaction (PCR) targeting thermonuclease (nuc) and mecA gene. A total of 115 bovine raw milk samples were collected and screened for the presence of S. aureus and MRSA. The samples were processed by standard conventional procedures for isolation of the S. aureus organism. Conventional culture method which include, Brain heart infusion broth with 10% sodium chloride followed by direct plating on Baird-Parker agar (BP) at 37° C for 24-48 hours. Molecular characterization of the isolates was done by PCR targeting thermonuclease (nuc) gene for S. aureus and for Methicillin Resistant Staphylococcus aureus (mecA) gene was used. Out of 115 milk samples 52/115 (45.22%) samples were found positive for S. aureus by conventional culture method. DNA was extracted from all the presumptive positive isolates. PCR targeting nuc gene for S. aureus and mecA gene for MRSA was carried out and the results showed that out of 48/52 (92.31%) for nuc gene and 39/52 (75%) for mecA gene were positive. This study showed that the prevalence of S. aureus and MRSA in bovine milk can play a role in zoonotic transmission, and PCR can be used as one of the rapidly and highly sensitive tests for detection and classification of S. aureus and MRSA by targeting nuc and mecA gene.
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