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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(2): 66-77
DOI: http://dx.doi.org/10.20546/ijcmas.2016.502.007


Identification and Antibiogram of Various Gram Positive Bacterial Isolates from Pyogenic Samples by VITEK® 2 Compact System
Shivani Khullar*, Laxmi Rathore, P. K. Khatri, R. S. Parihar, Saroj Meena, Archana Bora, Vinod Maurya and Niranjan Sharma
Department of Microbiology, Dr. S. N. Medical College, Jodhpur, Rajasthan, India
*Corresponding author
Abstract:

Drug resistance is a burning issue for bacterial isolates and in- appropriate use of antibiotics is one of the most important factors that could affect the increasing patterns of resistance. A predictable bacterial profile and antibiotic sensitivity will be of great help for clinicians to start empirical treatment. Hence periodical monitoring of bacterial profile and their antibiotic susceptibility pattern is important. Various clinical samples were obtained from 70 subjects from various departments of Hospitals which are associated with Dr. S. N. Medical College, Jodhpur, Western Rajasthan using aseptic methods and were processed in the laboratory immediately by using standard microbiological procedures. The bacterial isolates were identified by Gram’s staining and Vitek-2 compact automated system. The AST pattern of the isolates was also obtained by Vitek-2 compact automated system. Culture positivity in males was60.00% and 40.00% in females (M:F=3:2).Analysis of samples by Vitek-2 revealed that Staphylococcus aureus (40%) was the most common bacterial isolate followed by Enterococcus spp. (37.14%) and CONS (Coagulase negative Staphylococcus aureus) (22.85%). Among S. aureus16 (57.14%) were found to be Methicillin resistant Staphylococcus aureus (MRSA). The AST pattern showed that Tigecycline (58.33%), Nitrofurantoin (45.96%) and Vancomycin (40.36%) were the most susceptible drugs for the above isolates Changing antimicrobial resistance pattern poses challenge in treating pyogenic infections. It is very important to reduce frequent misuse; inadequate dosages and easy availability of antimicrobials to avoid antibiotic resistance. This study will thereby guide the clinician in choosing appropriate antibiotics which not only contributes to better treatment but their judicious use will also help in preventing emergence of resistance to the drugs which are still sensitive. Identification and AST pattern of bacterial isolates by Vitek-2 compact system is of great help to the clinicians as compared to the standard methods used, the use of Vitek-2 compact system would reduce the turnaround time by at least 16 hrs.


Keywords: Antimicrobial susceptibility, Vitek-2, Pyogenic samples, Antibiotic resistance, Staphylococcus, Gram positive cocci.
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How to cite this article:

Shivani Khullar, Laxmi Rathore, P. K. Khatri, R. S. Parihar, Saroj Meena, Archana Bora, Vinod Maurya and Niranjan Sharma. 2016. Identification and Antibiogram of Various Gram Positive Bacterial Isolates from Pyogenic Samples by VITEK® 2 Compact System.Int.J.Curr.Microbiol.App.Sci. 5(2): 66-77. doi: http://dx.doi.org/10.20546/ijcmas.2016.502.007