National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Banana is one of the important fruit crops and food source for million people in developing countries. In the study explants (Suckers) of two banana cultivars were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of BAP and Kinetin sole and in combination for shoot initiation and multiplication. The concentration of BAP alone tested was (0.5mg/l and 1.0mg/l) and kinetin (0.5mg/l and 1.0mg/l) whereas, the combination of 0.5mg/l BAP+0.5mg/l kin and 1.0mg/l BAP +0.5mg/l kin were used for shoot initiation. For multiplication, concentration of BAP(2.0,2.5 and 2.5mg/l) alone and in combination BAP+ Kin (1.5mg/l +1.5mg/l, 2.0mg/l +2.0mg/l and 2.5mg/l +2.5mg/l) were used. The rapid shoot initiation obtained from MS medium supplemented with the combination of 1.0mg/l BAP with 0.5mg/l kin (8 and 10 days) in both Poyo and Giant Cavendish respectively. The highest multiple shoot ( 6.0 and 4.5/explants), in Poyo and Giant Cavendish were observed on the MS medium fortified with 2.5mg/ l BAP+2.5mg/l kin and 2.0mg/l BAP +2.0mg/l Kin respectively. For root induction 1.5mg/l IBA and 1.5mg/l IAA each tested separately on MS medium. IBA showed best performance with 5.12 and 4.69 root/ plantlet after four weeks of inoculation in Poyo and Giant Cavendish respectively. After 12 weeks in vitro plantlets were transferred to green house for acclimatization where 82% and 88% survival rate was recorded in Poyo and Giant Cavendish respectively. The two cultivars studied exhibited variation in shoot initiation, shoot multiplication and rooting. Among the two cultivars tested Poyo found to be more responsive for in-vitro techniques. It had highest rate of shoot initiation and multiplication.
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