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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 3366-3375
DOI: https://doi.org/10.20546/ijcmas.2018.707.392


Studies on in vitro Micropropagation in Banana
Alango Kelta, Sunil T. Hajare and Akilu Banjaw
College of Natural and Computational Science, Dilla University, Dilla (Ethiopia)
*Corresponding author
Abstract:

Banana is one of the important fruit crops and food source for million people in developing countries. In the study explants (Suckers) of two banana cultivars were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of BAP and Kinetin sole and in combination for shoot initiation and multiplication. The concentration of BAP alone tested was (0.5mg/l and 1.0mg/l) and kinetin (0.5mg/l and 1.0mg/l) whereas, the combination of 0.5mg/l BAP+0.5mg/l kin and 1.0mg/l BAP +0.5mg/l kin were used for shoot initiation. For multiplication, concentration of BAP(2.0,2.5 and 2.5mg/l) alone and in combination BAP+ Kin (1.5mg/l +1.5mg/l, 2.0mg/l +2.0mg/l and 2.5mg/l +2.5mg/l) were used. The rapid shoot initiation obtained from MS medium supplemented with the combination of 1.0mg/l BAP with 0.5mg/l kin (8 and 10 days) in both Poyo and Giant Cavendish respectively. The highest multiple shoot ( 6.0 and 4.5/explants), in Poyo and Giant Cavendish were observed on the MS medium fortified with 2.5mg/ l BAP+2.5mg/l kin and 2.0mg/l BAP +2.0mg/l Kin respectively. For root induction 1.5mg/l IBA and 1.5mg/l IAA each tested separately on MS medium. IBA showed best performance with 5.12 and 4.69 root/ plantlet after four weeks of inoculation in Poyo and Giant Cavendish respectively. After 12 weeks in vitro plantlets were transferred to green house for acclimatization where 82% and 88% survival rate was recorded in Poyo and Giant Cavendish respectively. The two cultivars studied exhibited variation in shoot initiation, shoot multiplication and rooting. Among the two cultivars tested Poyo found to be more responsive for in-vitro techniques. It had highest rate of shoot initiation and multiplication.


Keywords: Banana, BAP, Kinetin, Micro propagation, In vitro shoot initiation, Shoot multiplication
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How to cite this article:

Alango Kelta, Sunil T. Hajare and Akilu Banjaw. 2018. Studies on in vitro Micropropagation in Banana.Int.J.Curr.Microbiol.App.Sci. 7(7): 3366-3375. doi: https://doi.org/10.20546/ijcmas.2018.707.392