National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The mechanism of benzimidazoles resistance is linked to single nucleotide polymorphisms (SNPs) on beta-tubulin isotype-1 gene. The three known SNPs responsible for BZ resistance are F200Y, F167Y and E198A on the beta-tubulin isotype-1. The present study was aimed to characterize beta-tubulin isotype-1 gene of Bunostomum trigonocephalum, for identifying variations on possible mutation sites. The adult parasites were collected from Mukteswar, Uttarakhand. The parasites were thoroughly examined morphologically and male parasites were subjected for RNA isolation. Complementary DNA (cDNA) was synthesised from total RNA using OdT. The PCR was performed using cDNA and self designed degenerative primers. The purified PCR amplicons were cloned into pGEMT easy vector and custom sequenced. The obtained sequences were analysed using DNA STAR, MEGA7.0 and Gene tool software. The deduced amino acid sequence showed 99> % homology with published B. trigonocephalum as well as closely related nematodes Ancylostomum caninum and Strongyles of equines. It is also showed 98-98.7% identity with Trichostrongylus species and 91.8-93.6% homology with other helminths like P.equorum, A. galli and F.hepatica. The information obtained from current study enlighten to investigate further related to benzimidazole resistance in B. trigonocephalum and formulate effective control strategy as this parasite is one of the most pathogenic strongyles of small ruminants.
Citations |
 |
 |
 |
 |
