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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 3351-3358
DOI: https://doi.org/10.20546/ijcmas.2018.707.390


Molecular Characterization of β-Tubulin Isotype-1 Gene of Bunostomum trigonocephalum
Ravi Kumar Khare1, A. Dixit3, G. Das4, A. Kumar1, K. Rinesh3, D.S. Khare4, D. Bhinsara1, Mohar Singh2, B.C. Parthasarathi2, P. Dipali2, M. Shakya5, J. Jayraw5, D. Chandra2 and M. Sankar1*
1Division of Temperate Animal Husbandry, ICAR- IVRI, Mukteswar, India
2IVRI, Izatnagar, India
3College of Veterinary Science and A.H., Rewa, India
4College of Veterinary Sciences and A.H., Jabalpur, India
5College of Veterinary Sciences and A.H., Mhow, India
*Corresponding author
Abstract:

The mechanism of benzimidazoles resistance is linked to single nucleotide polymorphisms (SNPs) on beta-tubulin isotype-1 gene. The three known SNPs responsible for BZ resistance are F200Y, F167Y and E198A on the beta-tubulin isotype-1. The present study was aimed to characterize beta-tubulin isotype-1 gene of Bunostomum trigonocephalum, for identifying variations on possible mutation sites. The adult parasites were collected from Mukteswar, Uttarakhand. The parasites were thoroughly examined morphologically and male parasites were subjected for RNA isolation. Complementary DNA (cDNA) was synthesised from total RNA using OdT. The PCR was performed using cDNA and self designed degenerative primers. The purified PCR amplicons were cloned into pGEMT easy vector and custom sequenced. The obtained sequences were analysed using DNA STAR, MEGA7.0 and Gene tool software. The deduced amino acid sequence showed 99> % homology with published B. trigonocephalum as well as closely related nematodes Ancylostomum caninum and Strongyles of equines. It is also showed 98-98.7% identity with Trichostrongylus species and 91.8-93.6% homology with other helminths like P.equorum, A. galli and F.hepatica. The information obtained from current study enlighten to investigate further related to benzimidazole resistance in B. trigonocephalum and formulate effective control strategy as this parasite is one of the most pathogenic strongyles of small ruminants.


Keywords: Benzimidazole resistance, Beta tubulin, Bunostomum trigonocephalum, Small ruminants
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How to cite this article:

Ravi Kumar Khare, A. Dixit, G. Das, A. Kumar, K. Rinesh, D.S. Khare, D. Bhinsara, Mohar Singh, B.C. Parthasarathi, P. Dipali, M. Shakya, J. Jayraw, D. Chandra and Sankar, M. 2018. Molecular Characterization of β-Tubulin Isotype-1 Gene of Bunostomum trigonocephalum.Int.J.Curr.Microbiol.App.Sci. 7(7): 3351-3358. doi: https://doi.org/10.20546/ijcmas.2018.707.390