National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Present study was conducted to evaluate the anti-apoptotic effect of caspase inhibitor (Z-DEVD-FMK) supplementation in buffalo bull semen. Z-DEVD-FMK was supplemented with Tris egg yolk extender @ 2, 4, 6, 10, 20 µM. Pre-freeze and post thaw samples were evaluated in terms of % individual motility, % viability, % HOS reactive sperms, status of mitochondrial membrane potential and status of sperm membrane phosphatidylserine. There was no significant effect after supplementation of caspase inhibitor on % sperm motility, % active mitochondria and % sperms with low PLA activity in pre-freeze semen samples. Supplementation of Z-DEVD-FMK did not improve sperm motility in post freeze semen sample also. However, there was significant (P < 0.05) improvement in terms of % live sperm, % HOS reactive Sperms, % active mitochondria and % sperms with low PLA activity in all supplementation doses of Z-DEVD-FMK in post freeze semen samples as compared to control (without supplementation). The percent live sperms and HOS reactive sperms were significantly (P < 0.05) higher @ 2 µM supplementation doses of Z-DEVD-FMK (i.e75.23% and 72.5%) as compared to other supplementation doses @ 4 µM (62.55% and 60.34%), 6 µM (60.38% and 58.76%), 10 µM (58.56% and 59.5%), 20 µM (61.36% and 60.56%) and control (58.8% and 50.4%). Live sperms and HOS reactive were significantly lower (P > 0.05) (46.36% and 44.56%) @ 20 µM as compared to control. However, % active mitochondria and % sperms with low PLA activity was significantly (P < 0.05) higher @ 20µM supplementation doses of Z-DEVD-FMK as compared to other supplementation doses (2µM, 4 µM, 6µM, 10µM) and control.
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