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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 3333-3340
DOI: https://doi.org/10.20546/ijcmas.2018.707.388


Minimization of Apoptosis like Changes by Supplementing Z-DEVD-FMK (CASPASE inhibitor) during Cryopreservation of Buffalo Bull Semen
Jasmer Dalal1*, AjeetKumar2, M. Honparkhe2, A.K. Singh2 and P.S. Brar2
1Department of Veterinary Gynaecology and Obstetrics, Lala Lajpat University of Veterinary and Animal University, Hisar, 125004, India
2Associate Professor, Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana 141 004, Punjab, India
*Corresponding author
Abstract:

Present study was conducted to evaluate the anti-apoptotic effect of caspase inhibitor (Z-DEVD-FMK) supplementation in buffalo bull semen. Z-DEVD-FMK was supplemented with Tris egg yolk extender @ 2, 4, 6, 10, 20 µM. Pre-freeze and post thaw samples were evaluated in terms of % individual motility, % viability, % HOS reactive sperms, status of mitochondrial membrane potential and status of sperm membrane phosphatidylserine. There was no significant effect after supplementation of caspase inhibitor on % sperm motility, % active mitochondria and % sperms with low PLA activity in pre-freeze semen samples. Supplementation of Z-DEVD-FMK did not improve sperm motility in post freeze semen sample also. However, there was significant (P < 0.05) improvement in terms of % live sperm, % HOS reactive Sperms, % active mitochondria and % sperms with low PLA activity in all supplementation doses of Z-DEVD-FMK in post freeze semen samples as compared to control (without supplementation). The percent live sperms and HOS reactive sperms were significantly (P < 0.05) higher @ 2 µM supplementation doses of Z-DEVD-FMK (i.e75.23% and 72.5%) as compared to other supplementation doses @ 4 µM (62.55% and 60.34%), 6 µM (60.38% and 58.76%), 10 µM (58.56% and 59.5%), 20 µM (61.36% and 60.56%) and control (58.8% and 50.4%). Live sperms and HOS reactive were significantly lower (P > 0.05) (46.36% and 44.56%) @ 20 µM as compared to control. However, % active mitochondria and % sperms with low PLA activity was significantly (P < 0.05) higher @ 20µM supplementation doses of Z-DEVD-FMK as compared to other supplementation doses (2µM, 4 µM, 6µM, 10µM) and control.


Keywords: Apoptosis like changes, Buffalo bull, Caspase inhibitor, Cryopreservation, Z-DEVD-FMK
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How to cite this article:

Jasmer Dalal, AjeetKumar, M. Honparkhe, A.K. Singh and Brar, P.S. 2018. Minimization of Apoptosis like Changes by Supplementing Z-DEVD-FMK (CASPASE inhibitor) during Cryopreservation of Buffalo Bull Semen.Int.J.Curr.Microbiol.App.Sci. 7(7): 3333-3340. doi: https://doi.org/10.20546/ijcmas.2018.707.388