International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:7, July, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 3319-3326

High in vitro Shoot Multiplication for Efficient Micropropagation of Banana Cv. Robusta (AAA)
Anita Kumari* and Harsh Kumar
Department of Agricultural Biotechnology and Molecular Biology, Dr. Rajendra Prasad Central Agricultural University, Pusa, Bihar, India- 848125
*Corresponding author

The cultivar Robusta forms the mainstay of commercial banana cultivation in India. An efficient micropropagation system with high multiplication rate will boost banana cultivation and assist banana based industries in the country. The shoot apices of banana cv. Robusta were cultured on two basal media MS and B5 supplemented with different concentrations and combinations of plant growth regulators (PGR). The tissue culture responses were observed for shoot multiplication and effect of subculture on rate of shoot multiplication till 8th subculture. The maximum frequency of multiple shoot formation (97.95%) and number of differentiated shoots per culture (27.25) was achieved on medium M11 (MS+1.14 μM IAA+19.97 μM BAP). The medium was further used for the multiplication of shoot buds upto eight subculture cycles. The mean number of differentiated shoots per culture was maintained upto 5th subculture cycle thereafter declined in 6th (-16.67%) to 8th (- 40.35%) subcultures. The in vitro developed shoots were rooted on medium M17 (MS + 4.92 µM IBA). The well rooted plantlets were acclimatized to field condition.

Keywords: Banana, Robusta, Shoot apices, Micropropagation, Subculture cycle and Multiple shoot formation

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How to cite this article:

Anita Kumari and Harsh Kumar. 2018. High in vitro Shoot Multiplication for Efficient Micropropagation of Banana Cv. Robusta (AAA).Int.J.Curr.Microbiol.App.Sci. 7(7): 3319-3326. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.