National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The cultivar Robusta forms the mainstay of commercial banana cultivation in India. An efficient micropropagation system with high multiplication rate will boost banana cultivation and assist banana based industries in the country. The shoot apices of banana cv. Robusta were cultured on two basal media MS and B5 supplemented with different concentrations and combinations of plant growth regulators (PGR). The tissue culture responses were observed for shoot multiplication and effect of subculture on rate of shoot multiplication till 8th subculture. The maximum frequency of multiple shoot formation (97.95%) and number of differentiated shoots per culture (27.25) was achieved on medium M11 (MS+1.14 μM IAA+19.97 μM BAP). The medium was further used for the multiplication of shoot buds upto eight subculture cycles. The mean number of differentiated shoots per culture was maintained upto 5th subculture cycle thereafter declined in 6th (-16.67%) to 8th (- 40.35%) subcultures. The in vitro developed shoots were rooted on medium M17 (MS + 4.92 µM IBA). The well rooted plantlets were acclimatized to field condition.
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