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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 3242-3253
DOI: https://doi.org/10.20546/ijcmas.2018.707.378


Development of Transgenic indica Rice with a Chimeric cry2AX1 Gene against Leaffolder, Cnaphalocrocis medinalis (Guenee)
G. Rajadurai1, S. Varanavasiyappan2, N. Balakrishnan2, V. Udayasuriyan2, D.Sudhakar2 and N. Natarajan1*
1Department of Agricultural Entomology
2Department of Plant Biotechnology, Agricultural college and Research Institute, Tamil Nadu Agricultural University, Coimbatore -641003, India
*Corresponding author
Abstract:

The insecticidal chimeric cry2AX1 gene was introduced into rice indica cultivar ASD16 with a view to impart resistance to a major lepidopteran pest, rice leaf folder. A total of 17 putative transgenic events were generated with two different constructs harbouringcry2AX1 gene and all of them were found to be positive for cry2AX1and hptII genes. The Cry2AX1 protein expression in T0transgenic rice events ranged from 0.021 to 0.093 μg/g of fresh leaf tissue. The detached leaf bit bioassay on selected transgenic plants against neonates of Cnaphalocrocis medinalis showed that the larval mortality ranged from 23.33 to 66.67 per cent in T0 transgenic plants.


Keywords: Transgenic rice, cry2AX1gene, Protein expression, Insect resistance, Leaf folder
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How to cite this article:

Rajadurai, G., S. Varanavasiyappan, N. Balakrishnan, V. Udayasuriyan, D. Sudhakar and Natarajan, N. 2018. Development of Transgenic indica Rice with a Chimeric cry2AX1 Gene against Leaffolder, Cnaphalocrocis medinalis (Guenee).Int.J.Curr.Microbiol.App.Sci. 7(7): 3242-3253. doi: https://doi.org/10.20546/ijcmas.2018.707.378