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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 3162-3168
DOI: https://doi.org/10.20546/ijcmas.2018.707.369


Primary and Secondary Metabolite Profiling, Unravelling the Antibiotic Susceptibility from Culture - Lysed Symbiotic Colonies- of Diazotroph Bacteria (Rhizobium leguminosarum) Isolated from Root Nodules of Dolichos Lab Lab.
G.V. Pavan Kumar*, G. Pooja, G.V. Nagaraju and Y. Malyadri
Koringa College of Pharmacy, Korangi-533461, Kakinada, Andhra Pradesh, India
*Corresponding author
Abstract:

This is the first study demonstrating antibiotic potential of symbiotic bacteria and their metabolite profiling from cultured bacterial endosymbionts associated with root nodules of Leguminaceae plant Dolichos lab lab. Our study has significant scope and it has never been reported. Indian farming area especially semi arid areas host varieties of Legumes with novel secondary metabolites producing organisms. The natural metabolites extracted from root nodule-derived bacteria pave novel therapeutic remedy against the pathogens like Bacillus subtilis, Escherichia coli and fungal species Aspergillus niger when most of them are emerged as extreme drug resistant superbugs. In recent study the bacteria isolated from the root nodule were cultured by standard microbiology techniques. Most of the bacteria isolated were found to be Symbiotic nitrogen fixation of Gram-negative, motile, Rhizobium leguminosarum. The potential isolate LNRLS9 was mass cultured for extraction of primary and secondary metabolites. Preliminary screening for identification of Bacterial metabolites by chemical tests was performed using reagents. Ethyl acetate extract prepared from the lysed culture of the isolate was analyzed for antibiotic activity. The results of this study suggested that secondary metabolites produced by Rhizobium leguminosarum sp. (LNRLS9) could be used as a lead to control bacterial pathogens.


Keywords: Rhizobium leguminosarum sp. LNRLS9, metabolites, Antimicrobial activity,
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How to cite this article:

Pavan Kumar, G.V., G. Pooja, G.V. Nagaraju and Malyadri, Y. 2018. Primary and Secondary Metabolite Profiling, Unravelling the Antibiotic Susceptibility from Culture - Lysed Symbiotic Colonies- of Diazotroph Bacteria (Rhizobium leguminosarum) Isolated from Root Nodules of Dolichos Lab Lab..Int.J.Curr.Microbiol.App.Sci. 7(7): 3162-3168. doi: https://doi.org/10.20546/ijcmas.2018.707.369