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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 3127-3134
DOI: https://doi.org/10.20546/ijcmas.2018.707.365


Biotransformation of Terfenadine to Fexofenadine by Absidia corymbifera ATCC 14058
Tankeswar Nath*, Manab Bikash Gogoi and Pranab Kumar Nath
DBT-AAU Centre, Assam Agricultural University, Jorhat-785013, India
*Corresponding author
Abstract:

Fexofenadine is an important medicament having significant antihistaminic activity. It is currently being prepared by chemical route which is in numerous stages of development with a yield of less than 10%. In this study, bio-transformation of terfenadine to fexofenadine was investigated using few microbial strains. Among the microbes studied, Absidia corymbifera ATCC14058 showed the highest conversion efficiency in a base line study. Therefore, process conditions conducive to conversion of terfenadine to fexofenadine were optimized using this strain. The growing cell-masses of this strain showed the maximum molar conversion of 99.19% and 94.76% at 200ppm and 500ppm substrate concentration, respectively. Above 500ppm substrate concentration, molar conversion efficiency found to be decreased considerably with increased concentration of substrate. However, the split-dose addition of substrate showed better conversion than the single dose addition. Again, growing cell-catalysts showed the highest substrate conversion over the resting cell-catalysts and cell-free extract. This strain also showed the ability to convert terfenadie to fexofendine in biphasic solvent systems. Among the different biphasic systems, the Ethanol-water and Dimethyl formamide (DMF)-water systems showed reasonably good results. The microbial bioconversion of terfenadine to fexafenadine with biphasic system is a new approach and carries greater promise for further research.


Keywords: Absidia corymbifera ATCC 14058, Terfenadine, Fexofenadine, Biotransformation, Molar conversion
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How to cite this article:

Tankeswar Nath, Manab Bikash Gogoi, Pranab Kumar Nath. 2018. Biotransformation of Terfenadine to Fexofenadine by Absidia corymbifera ATCC 14058Int.J.Curr.Microbiol.App.Sci. 7(7): 3127-3134. doi: https://doi.org/10.20546/ijcmas.2018.707.365