National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Fexofenadine is an important medicament having significant antihistaminic activity. It is currently being prepared by chemical route which is in numerous stages of development with a yield of less than 10%. In this study, bio-transformation of terfenadine to fexofenadine was investigated using few microbial strains. Among the microbes studied, Absidia corymbifera ATCC14058 showed the highest conversion efficiency in a base line study. Therefore, process conditions conducive to conversion of terfenadine to fexofenadine were optimized using this strain. The growing cell-masses of this strain showed the maximum molar conversion of 99.19% and 94.76% at 200ppm and 500ppm substrate concentration, respectively. Above 500ppm substrate concentration, molar conversion efficiency found to be decreased considerably with increased concentration of substrate. However, the split-dose addition of substrate showed better conversion than the single dose addition. Again, growing cell-catalysts showed the highest substrate conversion over the resting cell-catalysts and cell-free extract. This strain also showed the ability to convert terfenadie to fexofendine in biphasic solvent systems. Among the different biphasic systems, the Ethanol-water and Dimethyl formamide (DMF)-water systems showed reasonably good results. The microbial bioconversion of terfenadine to fexafenadine with biphasic system is a new approach and carries greater promise for further research.
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