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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 3080-3088
DOI: https://doi.org/10.20546/ijcmas.2018.707.359


Isolation, Molecular Characterization and Antimicrobial Resistance Patterns of Four Different Vibrio Species Isolated from Fresh Water Fishes
Y. Suresh, N. Subhashini, Ch. Bindu Kiranmayi*, K. Srinivas, V. Prasastha Ram, G. Chaitanya, B. Swathi Vimala and T. Srinivasa Rao
Department of Veterinary Public Health and Epidemiology, NTR College of Veterinary Science, Gannavaram, Sri Venkateswara Veterinary University (SVVU), Tirupati-517 502, Andhra Pradesh, India
*Corresponding author
Abstract:

Vibrio species are the major food borne pathogens commonly associated with aquatic food poisonings and lead to food-borne outbreaks. In this present study, out of 105 fresh water fish (Catlacatla) samples collected, 87 (82.85%) were found positive for Vibrio species. Out of 87, 6 (6.9%), 2 (2.3%), 4 (4.6%) and 3 (3.45%) isolates were found to be V. parahaemolyticus and V. vulnificus, V. alginolyticus and V. cholerae respectively by mPCR. The 15 different Vibrio species were subjected to antibiogram studies including ESBL detection. Antibiotics like ampicillin, penicillin, gentamycin, amikacin, tetracycline, ceftazidime, streptomycin and co-trimoxazole were used for antibiogram profile. Out of 15 isolates, 5 isolates were found positive for ESBLs by both phenotypic and molecular methods. Out of 5ESBL (only TEM gene) positive isolates, 2 (33.33%), 1 (50%), 1 (25%) and 1 (33.33%) were from V. parahaemolyticus, V. vulnificus, V. alginolyticus and V. cholera respectively by mPCR.


Keywords: Vibrio vulnificus, V. parahaemolyticus, V. alginolyticus, V. cholerae, Freshwater fishes, Extended Spectrum Beta-Lactamases
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How to cite this article:

Suresh, Y., N. Subhashini, Ch. Bindu Kiranmayi, K. Srinivas, V. Prasastha Ram, G. Chaitanya, B. Swathi Vimala and Srinivasa Rao, T. 2018. Isolation, Molecular Characterization and Antimicrobial Resistance Patterns of Four Different Vibrio species Isolated from Fresh Water Fishes.Int.J.Curr.Microbiol.App.Sci. 7(7): 3080-3088. doi: https://doi.org/10.20546/ijcmas.2018.707.359