International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:7, July, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 2800-2806

Molecular Diversity of Azotobacter sp. Isolates from Rhizosphere of Tropical Tasar Silkworm Host Plants
Manjappa, I.G. Prabhu, Niranjan Kumar and A.K. Sinha
Central Tasar Research and Training Institute, Ranchi, Jharkhand, India
*Corresponding author

Azotobacter is an aerobic, free living bacteria fixes atmospheric nitrogen in soil besides producing plant growth promoting substance. Eight Azotobacter isolates were isolated and purified from different tasar rearing regions of West Singhbhoom, Jharkhand on Jenson media by using serial dilution technique. Molecular diversity among these isolates was analysed using RAPD primers. Of the 20 decamer primers screened, 10 primers amplified clear and distinguishable fragments and yielded a total of 103 fragments, of which 101 fragments are polymorphic (98%). Unweighted neighbour joining dendrogram based on Dice indices divides eight Azotobacter isolates in two major clusters. Dissimilarity matrix showed a maximum difference of 76% between the isolate from Hatghamaria (Azo 21-1) and Talaburu (Azo 29-1) and minimum difference of 39% between the isolates derived from the same region, Kharswan (Azo 14 and Azo 15-1). Thus RAPD marker analysis was found to be easy and rapid in assessing diversity among Azotobacter isolates.

Keywords: Molecular diversity, Azotobacter sp., Rhizosphere, Tasar Silkworm

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How to cite this article:

Manjappa, I.G. Prabhu, Niranjan Kumar and Sinha, A.K. 2018. Molecular Diversity of Azotobacter sp. Isolates from Rhizosphere of Tropical Tasar Silkworm Host Plants.Int.J.Curr.Microbiol.App.Sci. 7(7): 2800-2806. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.