International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:7, July, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 2687-2692

In vitro Screening of Bacillus subtilis Isolates against Sclerotium rolfsii Cause for Collar Rot of Chilli
Rajkumar1*, M.K. Naik1, Y.S. Amaresh1 and G. Chennappa2
1Department of Plant Pathology, University of Agricultural Sciences, Raichur, India
2Department of Processing and Food Engineering, University of Agricultural Sciences, Raichur- 584104, India
*Corresponding author

Bacillus subtilis a gram positive, endospore forming bacteria play a major role in biocontrol and PGPR activities. Thirty isolates of B. subtilis were obtained from different rhizosphere soil samples from different parts of North Eastern Karnataka region. All the isolates were rod shaped, positive for gram reaction, endospore, oxidase, catalase, starch hydrolysis, negative for indole, KOH test and green coloured colonies were grown on Hichrome Bacillus agar medium. Thirty B. subtilis isolates were screened in vitro against S. rolfsii. The isolates showed different levels of inhibition of mycelia growth of S. rolfsii. Among different isolates BS16 inhibited maximum mycelial growth 64.04 per cent followed by BS 30 (11.98 %) and minimum inhibition of mycelial growth was observed in case of BS17 (11.98 %) compared to check isolate with 47 per cent inhibition of mycelial growth of S. rolfsii. The B. subtilis strains were isolated, identified and used in this present study is a promising natural bioagent which can be considered as an alternative to chemical pesticides in chilli disease management strategies and also used in integrated disease management.

Keywords: subtilis, Biocontrol, PGPR, Per cent inhibition, S. rolfsii

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How to cite this article:

Rajkumar, K., M.K. Naik, Y.S. Amaresh and Chennappa, G. 2018. In vitro Screening of Bacillus subtilis Isolates against Sclerotium rolfsii Cause for Collar Rot of Chilli.Int.J.Curr.Microbiol.App.Sci. 7(7): 2687-2692. doi:
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