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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 2656-2668
DOI: https://doi.org/10.20546/ijcmas.2018.707.312


Methods of Plant Transformation- A Review
Keshavareddy1*, A.R.V. Kumar1 and Vemanna S. Ramu2
1Department of Entomology, 2Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru-560065, Karnataka, India
*Corresponding author
Abstract:

Plant transformation is now a core research tool in plant biology and a practical tool for transgenic plant development.  There are many verified methods for stable introduction of novel genes into the nuclear genomes of diverse plant species.  As a result, gene transfer and regeneration of transgenic plants are no longer the factors limiting the development and application of practical transformation systems for many plant species.  However, the desire for higher transformation efficiency has stimulated work on not only improving various existing methods but also in inventing novel methods.  The most published techniques for gene transfer into plant cells were dismissed as either disproven or impractical for use in routine production of transgenic plants.  In many laboratories, virtually all the transformation work relies on particle bombardment with DNA coated microprojectiles or Agrobacterium mediated transformation for gene transfer to produce transgenic plants from a range of plant species.


Keywords: Transformation, Transgenic, Gene, Agrobacterium, Particle bombardment
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How to cite this article:

Keshavareddy, G., A.R.V. Kumar and Vemanna S. Ramu. 2018. Methods of Plant Transformation- A ReviewInt.J.Curr.Microbiol.App.Sci. 7(7): 2656-2668. doi: https://doi.org/10.20546/ijcmas.2018.707.312