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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 2588-2597
DOI: https://doi.org/10.20546/ijcmas.2018.707.303


Assessment of Mutation in Marigold (Tagetes erecta L.) using Morphological and Molecular Markers
Jayoti Majumder1*, S. K. Singh1 and Manjusha Verma2
1ICAR- Indian Agricultural Research Institute, New Delhi-110012, India
2ICAR-National Bureau of Plant Genetic Resource, New Delhi-110012, India
*Corresponding author
Abstract:

Variability caused by induced mutations is not essentially different from variability caused by spontaneous mutation during evolution. Although marigold (Tagetes spp.) is an important commercial crop the major breeding obstacle is non-availability of known sources for creating a novelty. Therefore, in vivo grown seedlings and in vitro raised proliferated cultures of marigold cultivar Pusa Narangi Gainda were subjected to gamma-irradiation to develop mutant populations. 11 Mutants were grown in the M1 generation and variants screened based on yield and morphological characters. The number of flowers per plant was recorded the maximum in both the parents i.e. Parent (53.24) followed by in vivo raised mutant v4 (40.78). The v4 exhibited maximum average flower diameter (5.71 cm) with equivalence to Parent (5.71 cm). The maximum petal width among the mutants of M1 was observed in vitro raised mutant v8 (12.55 mm). After screening the mutants through the morphology, genomic DNA from leaf samples was evaluated by six identified putative mutants (pm1-6) in PCR amplification using RAPD primers and the amplified DNA fragments from mutants were compared with their respective controls. Single marker and stepwise regression analysis were carried out in relation to percent variability indicate that the alleles OPA 10, markers are strongly associated with mutant identification. On the basis of dendogram generated, Putative mutant 3 shows highest dissimilarity than the parent (0.24). The Putative mutants 5 and 6 were found to be similar with each other (0.47) but were distinct from parent (0.44)


Keywords: Mutation in Marigold, Tagetes erect
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How to cite this article:

Jayoti Majumder, S. K. Singh and Manjusha Verma 2018. Assessment of Mutation in Marigold (Tagetes erecta L.) using Morphological and Molecular MarkersInt.J.Curr.Microbiol.App.Sci. 7(7): 2588-2597. doi: https://doi.org/10.20546/ijcmas.2018.707.303