National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Variability caused by induced mutations is not essentially different from variability caused by spontaneous mutation during evolution. Although marigold (Tagetes spp.) is an important commercial crop the major breeding obstacle is non-availability of known sources for creating a novelty. Therefore, in vivo grown seedlings and in vitro raised proliferated cultures of marigold cultivar Pusa Narangi Gainda were subjected to gamma-irradiation to develop mutant populations. 11 Mutants were grown in the M1 generation and variants screened based on yield and morphological characters. The number of flowers per plant was recorded the maximum in both the parents i.e. Parent (53.24) followed by in vivo raised mutant v4 (40.78). The v4 exhibited maximum average flower diameter (5.71 cm) with equivalence to Parent (5.71 cm). The maximum petal width among the mutants of M1 was observed in vitro raised mutant v8 (12.55 mm). After screening the mutants through the morphology, genomic DNA from leaf samples was evaluated by six identified putative mutants (pm1-6) in PCR amplification using RAPD primers and the amplified DNA fragments from mutants were compared with their respective controls. Single marker and stepwise regression analysis were carried out in relation to percent variability indicate that the alleles OPA 10, markers are strongly associated with mutant identification. On the basis of dendogram generated, Putative mutant 3 shows highest dissimilarity than the parent (0.24). The Putative mutants 5 and 6 were found to be similar with each other (0.47) but were distinct from parent (0.44)
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