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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 1865-1878
DOI: https://doi.org/10.20546/ijcmas.2018.707.222


Zinc Oxide Nano Particle (ZnO NPs) and Innate Immunity: New Perspectives in Immunotoxicity
Jitendra Kumar1*, Sonia2, Murlidhar Mitra3, Vaibhav Purwar4, Kavia Rani1,Hanuman P. Yadav5 and Gautam Kaul1
1Animal Biochemistry Division, ICAR- National Dairy Research Institute,
Karnal (Haryana)-13200, India
2Dairy Chemistry Division, ICAR- National Dairy Research Institute, Karnal (Haryana)-132001, India
3Department of Chemistry, IIT- Banaras Hindu University (BHU), Varanasi-221005,
Uttar Pradesh, India
4Livestock Production and Management, Division, ICAR- National Dairy Research Institute, Karnal (Haryana)-13200, India
5Veterinary Gynecology and Obstetrics Division ICAR-National Dairy Research Institute, Karnal (Haryana)-132001, India
*Corresponding author
Abstract:

In the contemporary, application of zinc oxide nanoparticles (ZnO NPs) is growing exponentially in food industry and allied sciences. Hence public concern about their potential adverse effect is increasing. ZnO NPs with their unique physiochemical properties are thought to underlie their exploitable immunological activities. A study was conducted to investigate effects of ZnO NPs with innate immune cells. To achieve this aim, amongst three experimental groups, two group received (5.0 and 50 mg/kg b.w), ZnO NPs was dispensed, subsequent analysis of the body, and different organs weight, cytotoxicity were done using (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazoliumbromide (MTT), Water-soluble tetrazolium salts (WST), Nitric oxide (NO), phagocytosis. The levels of NO yield, phagocytosis, and weight of spleen increased (*P<0.05) due to ZnO NPs at dose of 50 mg/kg b.w., which caused adverse effect on functionality of macrophages. ZnO NPs release of Zn 2+ ions and produced reactive oxygen species and caused dose dependent toxicity. The results demonstrate that the high dose of ZnO NPs caused toxicity which is analysis by body and liver, kidney, and spleen weight, cell viability (MTT and WST), nitric oxide (NO) level, phagocytosis assay. The levels of NO production, phagocytic activity, and spleen weight increased significant (*P< 0.05) while decrease in NO production and phagocytosis was observed. ZnO NPs (5.0 mg/kg b.w) caused significant increased phagocytosis, cell viability, therefore high at dose ZnO NPs caused immunocytotoxicity. Overall, nanotoxicology of ZnO NPs and their effect requires more study to fill up gap of immunotoxicity.


Keywords: Zinc oxide nanoparticle, Macrophages, Nitric oxide, Immunotoxicity, Phagocytosis
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How to cite this article:

Jitendra Kumar, Sonia, Murlidhar Mitra, Vaibhav Purwar, Kavia Rani, Hanuman P. Yadav and Gautam Kaul. 2018. Zinc Oxide Nano Particle (ZNO NPs) and Innate Immunity: New Perspectives in Immunotoxicity.Int.J.Curr.Microbiol.App.Sci. 7(7): 1865-1878. doi: https://doi.org/10.20546/ijcmas.2018.707.222