National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Pseudomonas aeruginosa and Acinetobacter species have emerged as important nosocomial pathogens. Carbapenems had been the drugs of choice for penicillin & cephalosporin resistant Pseudomonas and Acinetobacter species infections. However, this scenario has changed with the emergence of Metallo-beta-lactamase (MBL) producing strains as these enzymes hydrolyze all beta-lactams, thereby, increasing patient morbidity and mortality. This situation prompts early and accurate detection of MBL producers. Hence the present study was done to phenotypically evaluate the prevalence of MBL production among 235 clinical isolates of Pseudomonas aeruginosa (183) and Acinetobacter species (52). The antimicrobial susceptibility testing was done by Kirby Bauer disk diffusion method. About 26.4% were found to be resistant to carbapenems tested. These screen positive isolates gave results of positive MBL production among 79.0% and 59.7% isolates by imipenem-EDTA combined disk test and modified Hodge test respectively. The prevalence of MBL production was found to be 21.9% and 17.3% among isolates of Pseudomonas aeruginosa and Acinetobacter species respectively. The prevalence was found to be higher among isolates from inpatients (26.1%) in comparison to those from outpatients (9.5%). Most of the MBL producers were isolated from pus samples (33.7%), followed by sputum (18.6%). The in vitro antimicrobial susceptibility profile of MBL producers showed that they were multidrug resistant, being 100% sensitive only to colistin and polymyxin B. To conclude, detection of MBL producers should be routinely done in all microbiological laboratories along with implementation of strict infection control policies and antibiotic stewardship for better patient management.
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