International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:7, July, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 1713-1722

Effect of Media, Temperature, Light, pH and Nutrient Source on Growth and Development of Bipolaris oryzae Causing Brown Leaf Spot of Paddy
Channakeshava and N.S. Pankaja
1Department of Plant Pathology, College of Agriculture, V.C. Farm, Mandya, India
*Corresponding author

The pathogen Bipolaris oryzae was subjected to different cultural conditions viz., media, temperature, pH, light and nutrient source under in vitro conditions. The maximum radial growth of 84.83 mm was recorded on paddy leaf extract agar followed by potato dextrose agar (61.33 mm). On this medium, the colony appeared greyish-white to dark brown, thick, leathery slightly raised and profuse mycelia with brown colored conidia. Similarly on liquid media, maximum dry mycelial weight of 113.06 mg was recorded on paddy leaf extract broth followed by potato dextrose broth (98.28 mg).Maximum radial growth of 70.67 mm,62.83 mmand68.00 mm was recorded at 30°C,25°C and complete dark regime, respectively. Maximum dry mycelial weight of 113.0 mg was recorded at pH 7.0 followed by 103.0 mg at pH 7.5 and 97.32 mg at pH 6.5.Among the different carbon and nitrogen sources maximum growth was recorded in glucose (89.57, 102.86, 112.73 and 128.88 mg)and ammonium peptone (79.10, 97.43, 103.61 and 115.24 mg) at 0.5, 1.0, 1.5 and 2.0% concentration respectively.

Keywords: Media, Temperature, pH, Light and nutrition, Radial growth and dry mycelial weight

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How to cite this article:

Channakeshava, C. and Pankaja, N.S. 2018. Effect of Media, Temperature, Light, pH and Nutrient Source on Growth and Development of Bipolarisoryzae Causing Brown Leaf Spot of Paddy.Int.J.Curr.Microbiol.App.Sci. 7(7): 1713-1722. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.