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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 2027-2033
DOI: https://doi.org/10.20546/ijcmas.2018.707.239


Effect of Various Surface Sterilant on Contamination and Callus Regeneration of Ashoka (Saraca asoca Roxb. De Wilde) from Leaf Segment Explant
Sandeep Rout* and Neelam Khare
College of Forestry, Sam Higginbottom University of Agriculture Technology & Sciences, Allahabad-211007, Uttar Pradesh, India
*Corresponding author
Abstract:

The present experiment was conducted to find out the most suitable surface sterilant and timing for controlling contamination in the leaf segment explants of Ashoka (Saraca asoca Roxb. De Wilde) using MS as basal medium with BAP (2.0 mg/l) and NAA (0.5mg/l). Among the various sterilants and timing the explants sterilized with 0.1% HgCl2 for 15 min + 1% NaOCl for 2 min significantly reduced the percentage of fungal contamination (6.67%), maximum aseptic culture (93.33%). Callus percentage maximum was recorded in T19 (0.1% HgCl2 for 15 min + 1% NaOCl for 2 min) (93.33%) and callus spread (2.33×1.00 cm) was recorded in similar treatment. In vitro propagation of this high valued medicinal tree is of great importance for the mass supply of disease free planting materials.


Keywords: Callus, Contamination, Regeneration, Sterilant
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How to cite this article:

Sandeep Rout and Neelam Khare. 2018. Effect of Various Surface Sterilant on Contamination and Callus Regeneration of Ashoka (Saraca asoca Roxb. De Wilde) from Leaf Segment Explant.Int.J.Curr.Microbiol.App.Sci. 7(7): 2027-2033. doi: https://doi.org/10.20546/ijcmas.2018.707.239