National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present experiment was conducted to find out the most suitable surface sterilant and timing for controlling contamination in the leaf segment explants of Ashoka (Saraca asoca Roxb. De Wilde) using MS as basal medium with BAP (2.0 mg/l) and NAA (0.5mg/l). Among the various sterilants and timing the explants sterilized with 0.1% HgCl2 for 15 min + 1% NaOCl for 2 min significantly reduced the percentage of fungal contamination (6.67%), maximum aseptic culture (93.33%). Callus percentage maximum was recorded in T19 (0.1% HgCl2 for 15 min + 1% NaOCl for 2 min) (93.33%) and callus spread (2.33×1.00 cm) was recorded in similar treatment. In vitro propagation of this high valued medicinal tree is of great importance for the mass supply of disease free planting materials.
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