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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(9): 419-430
DOI: http://dx.doi.org/10.20546/ijcmas.2016.509.045


Isolation, Characterization and Description of Hydrocarbon Degrading Thermophilic Aeribacillus pallidus Strain MCM B-882 from Oil Reservoirs, India
Mahesh Chitrakoti*
Vidya Pratishthan’s School of Biotechnology, Baramati, India
*Corresponding author
Abstract:

Thermophilic alkalitolerant bacterial strain MCM B-882 was isolated from oil well formation water in India. MCM B-882 was Gram variable, aerobic, motile, endospore forming and rod shaped. The MCM B-882 cells were occurring either solitarily or arranged in pairs and are 0.38 (0.33-0.47) x 2.75 (1.52-2.88) μm in size. MCM B-882 grows in the pH range of 6.0-10.0 and optimally at 7.0; temperature range of 45 – 65°C and optimally at 60°C; as well as in salt (NaCl) range of 1- 4% and optimally at 2%. The fatty acid spectrum of MCM B-882 showed isopentadecanoic, palmitic, and  isoheptadecanoic acids were the predominant species. The 16S rDNA analysis revealed that strain MCM B-882 is closely affiliated with genus Aeribacillus as Aeribacillus pallidus. The G+C content of it’s DNA was 42.24 mol%. There is significant difference in  physiological properties, fatty acid profiles, G+C content between Aeribacillus pallidus MCM B 882 and reported phylogenetically closest Aeribacillus pallidus DSM 3670T  and also A. pallidus strain TD1. MCM B-882 can oxidizes hydrocarbons which was confirmed using INT assay. It produces biosurfactant when grown in presence of hydrocarbons. A. pallidus strain MCM B882 may be a good candidate to be used effectively in MEOR process as well as in bioremediation processes.


Keywords: Oil well, Formation water, Aeribacillus,INT  assay
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How to cite this article:

Mahesh Chitrakoti. 2016. Isolation, Characterization and Description of Hydrocarbon Degrading Thermophilic Aeribacillus pallidus Strain MCM B-882 from Oil Reservoirs, India.Int.J.Curr.Microbiol.App.Sci. 5(9): 419-430. doi: http://dx.doi.org/10.20546/ijcmas.2016.509.045