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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 2509-2515
DOI: https://doi.org/10.20546/ijcmas.2018.707.294


Effect of Surface Sterilization Time on Leaf Explants for Aseptic Culture in Anthurium adraeanum (Hort.) cv. Fire
Rashmita Toppo1* and Sashikala Beura2
1Floriculture and Landscaping, College of Agriculture, OUAT
2Floriculture and Landscaping, College of Agriculture and BTCC, OUAT
*Corresponding author
Abstract:

Anthurium is an important ornamental plant grown around the globe and also in India. Seed and vegetative propagation of this plant are tedious and very slow process and not practical when carried out on a large scale. Therefore, biotechnological approach such as in- vitro cloning is found to be a novel method for production of pathogen free true-to-type plantlets at a faster rate. In this study, a competent surface sterilization procedure was standardized for aseptic in-vitro culture from leaf explants of Anthurium adraeanum (Hort.) cv. Fire under in vitro conditions. The leaf explants were treated with 0.1% HgCl2 (2, 3, 4, 5, 6, 7 and 8 min) and control (distilled water) were cultured on MS media. The leaf explants treated for 5 minutes resulted in minimum fungal (0.00%) and bacterial (0.00%) contamination with maximum survival percentage (100%). The best surface sterilization time was taken into consideration for further sterilization explants and in-vitro culture in the MS Basal media supplemented with of BAP alone or in combination with NAA and 2,4-D along with control for production of pathogen free true-to-type plantlets in a faster rate.


Keywords: Anthurium, Sterilization, Survival, HgCl2
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How to cite this article:

Rashmita Toppo and Sashikala Beura. 2018. Effect of Surface Sterilization Time on leaf Explants for Aseptic Culture in Anthurium adraeanum (Hort.) cv. FireInt.J.Curr.Microbiol.App.Sci. 7(7): 2509-2515. doi: https://doi.org/10.20546/ijcmas.2018.707.294