National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Indole-3-acetic acid (IAA) is the main member of the auxin family that controls many important physiological processes including cell enlargement and division. In the present study Serratia spp. and Pseudomonas spp. were isolated from the soil samples of the Akola region. 10 soil samples from agriculture and garden of various locations were collected. A total of 25 isolates were obtained which included 10 Serratia spp. and 15 Pseudomonas spp. The isolates where then subjected for their ability to produce indole acetic acid by standard procedure using L-tryptophan. Results showed that the isolate P6 (Pseudomonas spp) and S7 (Serratia spp) have ability to produce IAA in significant amount. Further studies on optimization suggest that – IAA production was maximum at 40°C for S7 and 37° C for P6 at 72 hrs of incubation at pH 9 and 7 respectively. The Mannitol was found to be best carbon source for IAA production. The 1% and 2% tryptophan was found to be optimum for maximum IAA production for P6 and S7 respectively.
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