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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 2378-2387
DOI: https://doi.org/10.20546/ijcmas.2018.707.277


Identification, Isolation and Detection of Metallo Beta Lactamase Resistance in Acinetobacter Species from Various Clinical Samples in a Tertiary Care Hospital
Sundararajan Thangavel, Gomathi Manian and Neelaveni Ramasamy
Department of Microbiology, Govt. Mohan Kumaramangalam Medical College, Salem, Tamil Nadu, India
*Corresponding author
Abstract:

To isolate, identify and detect Carbapenem resistance producing Acinetobacter spp., and confirm Metallo Beta Lactamase ( MBL) production by phenotypic methods. This cross sectional study conducted in a tertiary care hospital for a period of 6 months from various clinical samples were identified using standard protocol. The MBL resistant strains of Acinetobacter species were identified by Kirby-Bauer disc diffusion methods and confirmed by phenotypic methods. Out of clinically significant isolates of Acinetobacter spp., 50 (67%) were Acinetobacter baumannii and 25 (33%) were Acinetobacter lwoffi. The antimicrobial susceptibility pattern revealed maximum resistance to Gentamycin (64%), Cotrimoxazole, Amikacin & Ciprofloxacin (40%) and Cefotaxime and Ceftazidime (36%). Sensitivity to Polymyxin B (100%) followed by Imipenem and Meropenem (90%). Among them 7 (9.3%.) isolates were MBL producers. Among the 7 isolates, CDDT was positive in 5(71%) isolates, DDST was positive in 3(43%) isolates. Acinetobacter baumannii were the most common isolate in this study. Difference in antimicrobial susceptibility poses a great problem in treating these infections. MBL production by these organisms leads to high morbidity and mortality and left with the only option of treating them by potentially toxic drugs like Colistin and Polymyxin B.


Keywords: Acinetobacter, Metallo Beta Lactamase (MBL), Combined Disc Diffusion Test (CDDT),
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How to cite this article:

Sundararajan Thangavel, Gomathi Manian, Neelaveni Ramasamy 2018. Identification, Isolation and Detection of Metallo Beta Lactamase Resistance in Acinetobacter Species from Various Clinical Samples in a Tertiary Care HospitalInt.J.Curr.Microbiol.App.Sci. 7(7): 2378-2387. doi: https://doi.org/10.20546/ijcmas.2018.707.277