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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 2225-2235
DOI: https://doi.org/10.20546/ijcmas.2018.707.261


Molecular Characterization of Coat Protein Gene of Blackgram Yellow Mosaic Virus (BGYMV) from Karnataka
G.U. Prema1* and K.T. Rangaswamy2
1Department of Plant Pathology, College of Agriculture, Vijaypur, UAS, Dharwad-586101
2Department of Plant Pathology, College of Agriculture, UAS, GKVK, Bangalore-560065
*Corresponding author
Abstract:

Yellow mosaic virus is the most destructive disease of urdbean causing 5-100 per cent yield loss. BGYMV belongs to genus Begomovirus of the family Geminiviridae transmitted by white fly (Bemisia tabaci Gennadius). Polymerase chain reaction of yellow mosaic virus infecting blackgram samples using MYMV-CP-F/MYMV-CP-R primers amplified the expected product of size 1000 bp from blackgram infected samples. Expected PCR products of size 1000 bp obtained were cloned, sequenced and assembled. The total number of sequences obtained from blackgram yellow mosaic was 880 bp with 106 bp of pre-coat protein region and 774 bp of coat protein region of blackgram yellow mosaic virus. 257 amino acid lengths were predicted after translation of the nucleotide sequences. The cluster phylogram based on pairwise and multiple sequence alignment of the nucleotide sequence of the CP gene of 8 isolates of MYMV and 13 isolates of MYMIV indicated that the present isolate causing blackgram yellow mosaic virus formed cluster with other known isolates of MYMV. Sequence comparisons indicated that BGYMV has the highest nucleotide sequence identity of about 98.7 per cent, 98.4 per cent and 98.3 per cent and with MYMV-Namakkal:MoB [DQ865201.1]; MYMV-Madurai:SB [AJ421642.1], MYMV-Tamil Nadu:MB [AJ132575.1] and MYMV-Maharashtra:SB [AF314530.1] isolates, respectively. The nucleotide sequence identity of BGYMV with MYMV ranged from 94.4-98.7 per cent. The nucleotide sequence identity of BGYMV with MYMIV ranged between 79-80.7 per cent. When the deduced amino acid sequence of individual proteins were compared with those of other begomoviruses, the maximum homology of 99.2 per cent and 98.8 per cent was noticed with MYMV-Tamil Nadu:MB [AJ132575.1]; MYMV-Maharashtra:SB [AF314530.1] and MYMV-Namakkal:MoB [DQ865201.1]; MYMV-Madurai:SB [AJ421642.1] isolates, respectively. The deduced amino acid identities of BGYMV with Mungbean yellow mosaic virus revealed identities between 99.2-95.7 per cent. Deduced amino acid sequence comparison revealed that BGYMV revealed identities ranged from 84-85.9 per cent with Mungbean yellow mosaic India virus at amino acid level. The results of the present study revealed that coat protein gene of yellow mosaic virus infecting blackgram (BGYMV-Hebbal-Bangalore) is a Mungbean yellow mosaic virus (MYMV) but not Mungbean yellow mosaic India (MYMIV) virus and it is a variant of mungbean yellow mosaic virus since it showed 94.4-98.7 per cent identity at nucleotide level with other MYMV isolates.


Keywords: Blackgram yellow mosaic virus (BGYMV), Coat protein, Characterization, Phylogenetic analysis, Nucleotide sequence identity, Amino acid identity
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How to cite this article:

Prema, G.U. and Rangaswamy, K.T. 2018. Molecular Characterization of Coat Protein Gene of Blackgram Yellow Mosaic Virus (BGYMV) from Karnataka.Int.J.Curr.Microbiol.App.Sci. 7(7): 2225-2235. doi: https://doi.org/10.20546/ijcmas.2018.707.261