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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 2187-2210
DOI: https://doi.org/10.20546/ijcmas.2018.707.258


Isolation, Characterization and Evaluation of Pink Pigmented Facultative Methylotrophs (Ppfms) Associated with Paddy
N.S. Nysanth1*, K.S. Meenakumari1, Elizabeth K. Syriac2 and P. Subha1
1Department of Agricultural Microbiology, College of Agriculture, Vellayani-695522, Thiruvananthapuram, Kerala Agricultural University, Kerala, India
2Department of Agronomy, College of Agriculture, Vellayani-695522, Thiruvananthapuram, Kerala Agricultural University, Kerala, India
*Corresponding author
Abstract:

Methylobacterium spp. are a group of bacteria known as pink-pigmented facultative methylotrophs (PPFMs) and they are capable of growing on single carbon compounds such as formate, formaldehyde, and methanol as well as on a variety of multicarbon compound having no carbon-carbon bonds. They are distributed ubiquitously in the plant phyllosphere and rhizosphere and have been isolated from many species of plants. Methylotrophs are known to play an important role in increasing crop yield and soil fertility. Methylobacterium is able to produce indole-3-acetic acid (IAA), suggesting that inoculation of PPFM bacteria could increase plant IAA concentrations and promote plant growth. The present programme envisages isolation, characterization and evaluation of Pink Pigmented Facultative Methylotrophs (PPFMs) associated with paddy. As part of the study conducted in the Department of Agricultural Microbiology, College of Agriculture, Vellayani, Thiruvananthapuram, Kerala, during 2015-2017, Pink Pigmented Facultative Methylotrophs (PPFMs) were isolated from the phyllosphere of paddy collected from different agro climatic conditions of Kerala by leaf imprint method using Ammonium Mineral Salt (AMS) agar media supplemented with 0.5% methanol and cycloheximide. In all, 46 isolates were obtained. The isolates were obtained from different districts of Kerala such as Thiruvananthapuram, Alappuzha and Palakkad including Attappadi hill tract. They were tentatively identified as PPFMs based on the characteristic pink pigmented colonies on AMS agar media with methanol as sole source of carbon and energy. All the 47 PPFM isolates including reference culture were found to produce IAA under in vitro conditions. However, it showed wide variations ranging from 6.74 to 33.35 µg mL-1 of culture filtrate. Maximum IAA production of 33.35µg mL-1 of culture was recorded by PPFM35. Paddy seeds [var. Jyothi (Ptb-39)] treated with PPFMs improved seed germination, biomass and seedling vigor index of paddy seedlings. Maximum germination percentage of 100 was recorded in seeds treated with PPFM35. The isolate PPFM22 treated seedlings recorded the highest seedling vigour index of 4756.36 whereas PPFM35 recorded seedling vigour index of 4250.00 over the control (3037.91). The root shoot ratio of seedlings showed significant increase when seeds were treated with PPFM isolates. Maximum root shoot ratio of 0.62 was observed when seeds were treated with PPFM26 and PPFM35 compared to control (0.33). The isolate PPFM35 was adjudged as superior isolate based on indole-3-acetic acid (IAA) production, maximum germination percentage, seedling vigour index and root shoot ratio. This isolate was identified as Methylobacterium populi based on morphological, biochemical and molecular characteristics.


Keywords: Pink pigmented facultative methylotrophs, Phyllosphere, paddy, Kerala
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How to cite this article:

Nysanth, N.S., K.S. Meenakumari, Elizabeth K. Syriac and Subha, P. 2018. Isolation, Characterization and Evaluation of Pink Pigmented Facultative Methylotrophs (PPFMS) associated with PaddyInt.J.Curr.Microbiol.App.Sci. 7(7): 2187-2210. doi: https://doi.org/10.20546/ijcmas.2018.707.258