International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:7, July, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 2148-2154

Genetic Diversity Analysis of Pleurotus spp. in Himachal Pradesh Using RAPD Fingerprints
Rishu Sharma*, B.M. Sharma and P.N. Sharma
Department of Plant Pathology, CSKHPKV, Palampur, H. P- 176062, India
*Corresponding author

Random amplified polymorphism DNA (RAPD) analysis was done to assess the diversity among 21 species/strains of Pleurotus. The morphologically similar species/ strains too gave a new account of the evolutionary process and taxonomy of mushrooms. A total of 150 10 mer primers were screened, out of which 10 primers viz. OPD-03, OPD-05, OPD-08, OPA-13, OPA-16, OPQ-15, OPQ-16 and OPQ-18, S-1461 and S-1462 produced consistent polymorphic banding pattern. The RAPD dendogram obtained by Unweighted Pair Group Method with Arithmetic Mean (UPGMA) programme revealed a high genetic diversity among the isolates of Pleurotus. Twenty one isolates were divided into three clusters using 16 per cent similarity as a cut-off point. The cluster I accommodated 17 isolates of different species, whereas cluster III contained one strain of P. fossulatus I (P8) and two strains of Pleurotus sp. III (P15) and Pleurotus sp. IV (P18). RAPD bands were scored as present (1) or absent (0) for all the Pleurotus isolates. Each band was assumed to represent a unique genetic locus. The pattern and extent of RAPD variation were analysed with respect to primer, polymorphic locus and isolate. Total number of amplified fragment and polymorphic fragment produced by 10 decamer primer was 141 and 109, respectively with a polymorphism percentage of 77.30.

Keywords: Pleurotus, RAPD, UPGMA, Tissue culture, Scoring

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How to cite this article:

Rishu Sharma, B.M. Sharma and Sharma, P.N. 2018. Genetic Diversity Analysis of Pleurotus spp. in Himachal Pradesh Using RAPD Fingerprints.Int.J.Curr.Microbiol.App.Sci. 7(7): 2148-2154. doi:
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