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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 1643-1649
DOI: https://doi.org/10.20546/ijcmas.2018.707.192


Prediction, Synthesis and Evaluation of an Antimicrobial Peptide from Goat Vitronectin (Vn)
Prasanta Kumar Koustasa Mishra*, Aditya Agrawal, Anil Gattani and Yapu Nijo
Division of Biochemistry, Indian Veterinary Research Institute, Bareilly, UP-243122, India
*Corresponding author
Abstract:

Antimicrobial resistance is an emerging challenge for health personnel and researchers. In such scenario, alternative strategies targeting novel ways to kill or inhibit growth of microbes are need of the hour. Antimicrobial peptide can be a promising candidate to deal with this crisis. Extracellular matrix protein vitronectin (Vn) is recently being established as a bactericidal agent against certain microbes. Caprine Vn has got few structural alterations as compare to human Vn. Here we have applied bioinformatics approaches to screen as well as characterise regions of goat Vn having antimicrobial peptide followed by synthesis of a predicted peptide and evaluation of the antimicrobial effect of the same against Escherichia coli DH5-α cells. The minimum bactericidal concentration (MBC) was determined by resazurin reduction test (RRT) and found to be 250 µg /107 E. coli cells. The peptide gave visible inhibition against the test organism.


Keywords: Antimicrobial peptide, Vitronectin, Escherichia coli DH5-α, MBC,
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How to cite this article:

Prasanta Kumar Koustasa Mishra, Aditya Agrawal, Anil Gattani, Yapu Nijo. 2018. Prediction, Synthesis and Evaluation of an Antimicrobial Peptide from Goat Vitronectin (Vn).Int.J.Curr.Microbiol.App.Sci. 7(7): 1643-1649. doi: https://doi.org/10.20546/ijcmas.2018.707.192