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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 1316-1321
DOI: https://doi.org/10.20546/ijcmas.2018.707.157


Comparative Jejunal Expression of TFRC gene in Pigs Differentially Adhesive to Diarrhoeagenic E. coli
Chandrakanta Rawat1*, Nihar Ranjan Sahoo2, Shivaji S. Wagh1,D. Verma Ankita3 and Kanti Raje4
1Division of Animal Genetics, ICAR- Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P-243122 India
2Livestock Production & Management Section, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly U.P-243122, India
3Department of Animal Genetics & Breeding, Col. of Vet. Sci. & A.H. Junagarh, Gujrat, India
4Division of Animal Nutrition, ICAR- Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P-243122, India
*Corresponding author
Abstract:

Diarrhoea in piglets due to Escherichia coli is an important problem in the pig farming industry. Adhesion of these bacteria to the epithelial cells of small intestine is an essential prerequisite for the incidence of diarrhoea. Among the putative candidate genes associated with adhesion pattern, TFRC (transferrin receptor) gene was localized on targeted region of SSC13 and considered as a positional candidate gene. The present investigation was conducted to evaluate the Indian desi pigs in terms of E. coli adhesion pattern (with Indian isolate) and study TFRC expression profile in different adhesion phenotypes. A total of 4 types of adhesion pattern were observed with different frequencies. RT-PCR analysis revealed that TFRC mRNA expression level was different across the differentially adhesive phenotypes. The increased level of TFRC expression indicates its role in immunity against E. coli mediated diarrhoea.


Keywords: Adhesion pattern, E. coli, Desi pig, TFRC, Jejunal expression profiling
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How to cite this article:

Chandrakanta Rawat, Nihar Ranjan Sahoo, Shivaji S. Wagh, D. Verma Ankita and Kanti Raje. 2018. Comparative Jejunal Expression of TFRC gene in Pigs Differentially Adhesive to Diarrhoeagenic E. coli.Int.J.Curr.Microbiol.App.Sci. 7(7): 1316-1321. doi: https://doi.org/10.20546/ijcmas.2018.707.157