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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 946-955
DOI: https://doi.org/10.20546/ijcmas.2018.707.114


Isolation and Characterization of Bacteria for Amylase Production under Solid State Fermentation Using Damaged Wheat as Substrate
Nadia Razdan* and G.S. Kocher
Department of Microbiology Punjab Agricultural University, Ludhiana-141004, India
*Corresponding author
Abstract:

A bacterial isolate (I5) along with Bacillus subtilis and Bacillus circulans was evaluated for extracellular amylase production under SmF and SSF conditions. In SmF (Temperature - 28±2°C, pH -7 and inoculum size-106 cfu ml-1, Time- 96 h), maximum saccharifying activity (SA) was observed with Bacillus subtilis (0.62 IU/ml/min) followed by isolate (I5) (0.58IU/ml/min) and Bacillus circulans (0.46IU/ml/min). Whereas dextrinizing activity (DA) was recorded with Bacillus circulans (1.69U/ml), followed by isolate (I5) (1.65U/ml) and Bacillus subtilis (1.02U/ml). In SSF(1:2 moisture content, incubation time -96 hours, pH-7inoculum size-106 cfu ml-1 Temp-37°C) using different substrates (potato starch, corn starch, raw wheat flour and rice starch) as carbon sources. Maximum saccharifying activity (23.10IU/ml/min) was expressed by Bacillus circulans in 1% rice starch at 28±2°C. Whereas highest dextrinizing activity was recorded with 1% corn starch (3.31U/ml). On the basis of the present study, Bacillus circulans being the most potential strain for maximum and cost effective alpha amylase enzyme production at appropriate conditions could be utilized as bio-inoculant in fermentation technology in sustainable manner.


Keywords: Dextrinizing, Saccharifying, Inoculum, Bio-inoculant, Fermentation
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How to cite this article:

Nadia Razdan and Kocher, G.S. 2018. Isolation and Characterization of Bacteria for Amylase Production under Solid State Fermentation Using Damaged Wheat as Substrate.Int.J.Curr.Microbiol.App.Sci. 7(7): 946-955. doi: https://doi.org/10.20546/ijcmas.2018.707.114