National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Fish acts as an intermediate host to many helminth parasites, including trematodes of public health significance. In this study, we screened freshwater fish samples (n=195) collected from different markets located in and around Kolkata, to isolate and identify larval stage of minute intestinal fluke, Centrocestus formosanus employing a fast and accurate molecular approach. Processing of fish samples through pepsin digestion method revealed an overall prevalence of 14.87 % infection in fish with encysted metacercariae of trematode, Centrocestus. The prevalence rate was highest in Mystus tengara (40.00%) followed by Puntius spp. (26.66%) and Awaous grammepomus (5.26 %), whereas screening fish species such as Labeo bata, Cirrhinus mrigala and Oreochromis niloticus did not harbor any such larval stage of this trematode. Polymerase chain reaction using oligonucleotide primer sets from extracted genomic DNA of these metacercariae revealed amplicon of approx. 492 bp closely resembling to Centrocestus. Phylogenesis showed maximum relationship with the Centrocestus spp. Therefore, implementation of control strategies, apart from awareness of public and adoption of good aquaculture practices by farmers, are required in order to minimize the risk of infection in fish.
Citations |
 |
 |
 |
 |
