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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 761-772
DOI: https://doi.org/10.20546/ijcmas.2018.707.093


Molecular Diversity of Wheat (Triticum aestivum L.) Genotypes Resistance to Rice Weevil (Sitophilus oryzae L.) Revealed by SSR Markers
Ekhlaque Ahmad1,2*, Saurabh Badoni1, Moin Akhtar1 and J.P. Jaiswal1
1Department of Genetics and Plant Breeding, G. B. Pant University of Agriculture and Technology, Pantnagar-263145 (Uttarakhand), India
2Zonal Research Station (Birsa Agricultural University), Chianki, Palamau-822102, India
*Corresponding author
Abstract:

The present investigation was carried out to analyse for the presence of α-amylase inhibitor gene which is responsible for resistance to rice weevil. A total of 30 simple sequence repeat (SSR) markers were used to study the genetic diversity among 30 wheat genotypes resistant to rice weevil. Among them, seventeen primers were found polymorphic on agarose gel and depicted the significant diversity among susceptible and resistant genotypes while rest thirteen primers were found monomorphic. The number of polymorphic bands per loci ranged from 1 to 3 with an average of 1.6 alleles per locus. A clear cut differentiation was exhibited among the genotypes. The average PIC value was 0.37 ranging from 0.17 (WMC120/ WMC76/WMC245) to 0.67 (WMC267), indicating diverse nature of the wheat genotypes and/or highly informative SSR markers used in this study. The analyzed wheat genotypes showed a good level of genetic variability for assessed quantitative, physio-chemical and molecular characters. Molecular markers linked with major genes for traits of interest which have been amplified by the primer set UCW108, a genic marker for GPC-B1. The range of similarity coefficient varied from 54% (PBN-51) to 74% (K 20). SAHN cluster analysis using UPGMA method separated the parental genotypes into four cluster groups, PBN 51 was positioned as single genotype in separate groups i.e., in cluster-I, K 50, K 76 and K 77 in cluster-II, K 2I and K 50 in cluster-III and K 20 in cluster-IV. In dendrogram, based on results of markers validated were able to diversify the resistant and susceptible parents, first group member PBN51 and fourth group member K20 found on the two distant groups, which were observed as most susceptible and resistant genotypes respectively, according to our controlled experiment on weevil infestation and molecular analysis done and hence, the study supports and correlates the result, obtained by these analysis. The present study also indicates that microsatellite markers able to access the genetic diversity among studied genotypes of wheat for weevil resistance.


Keywords: Wheat (Triticum aestivum L.), Molecular diversity, SSR marker, Weevil resistance
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How to cite this article:

Ekhlaque Ahmad, Saurabh Badoni, Moin Akhtar and Jaiswal, J.P. 2018. Molecular Diversity of Wheat (Triticum aestivum L.) Genotypes Resistance to Rice Weevil (Sitophilus oryzae L.) Revealed by SSR Markers.Int.J.Curr.Microbiol.App.Sci. 7(7): 761-772. doi: https://doi.org/10.20546/ijcmas.2018.707.093