National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was envisaged with the objective to isolate indigenous chromate tolerant bacteria from effluents and their subsequent utilization for chromium uptake or reduction. Samples were collected from two different Common Effluent Treatment Plants (CETPs), located in Ludhiana (sample 1) and Jalandhar (sample 2). In both the samples, chromium was found to be the dominant metal contaminant. A total of 10 morphologically distinct isolates were tested for their tolerance to chromium in terms of minimum inhibitory concentration (MIC) of Cr required for complete inhibition of growth. Four isolates (HM 2, HM 3, HM 15 and HM 16) showed maximum tolerance to chromium. There was no active uptake of Cr in sample 1 but considerable uptake was observed in sample 2. Chromium reduction efficiency was determined by S-diphenyl-carbazide (DPC) method, whereby complete reduction was observed with the standard culture (Shewanella putrefaciens) followed by 76.66% by HM 16 and 46.76% by HM 2 after 7 hours of incubation. Molecular characterization of most potent isolate (HM 16) was carried out using 16S rDNA based molecular method.
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