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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(9): 234-241
DOI: http://dx.doi.org/10.20546/ijcmas.2016.509.026


Antibiotic Properties of Aqueous and Methanol Extracts of Acalypha ciliata Leaves
C.A. Anosike*, O.F.C. Nwodo and E.B. Amadi
Department of Biochemistry, University of Nigeria, Nsukka, Enugu State, Nigeria  
*Corresponding author
Abstract:

This study demonstrated the antibiotic properties of aqueous and methanol extracts of Acalypha ciliata leaves. This was carried out using both gram-negative and gram-positive bacteria which included Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Salmonella typhi, Proteus mirabilis, Klebsiella pneumonia, and Streptococus pyrogenes. The aqueous extract of Acalypha ciliata leaves showed antibacterial activity ranging from 8.00 - 17.00±2.00mm, while that of methanol extract was from 9.00 - 20.00±4.00mm. The duration of antibacterial activity observed in aqueous extract ranged from 5.00 – 11.00± 2.00 days, while that of methanol extract ranged from 5.00 – 12.00±.00 days. The minimum inhibitory concentrations (MIC) of both methanol and aqueous extracts against the test bacteria were between 6.25 – 12.50±0.00mg/ml. However, the minimum bactericidal concentration (MBC) of both aqueous and methanol ranged from 9.38 – 25.00±0.00 mg/ml. The result of colony forming unit (CFU) showed that Klebsiella pnumoniae had the highest CFU value of 4.15 ±0.04 x 108cfu/ml while Proteus mirabilis had the least CFU             of 1.27±0.06 x 108cfu/ml. This research indicates that extracts of Acalypha ciliata leaves has broad spectrum antibacterial properties.


Keywords: Antibacterial activity,Acalypha ciliata, microorganism, Colony forming unit, MIC.
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How to cite this article:

Anosike, C.A., O.F.C. Nwodo and Amadi, E.B. 2016. Antibiotic Properties of Aqueous and Methanol Extracts of Acalypha ciliata LeavesInt.J.Curr.Microbiol.App.Sci. 5(9): 234-241. doi: http://dx.doi.org/10.20546/ijcmas.2016.509.026