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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Milk is considered as an important food material for consumers of all age groups. Albeit that, it can harbor a number of pathogens including Staphylococcus aureus which causes a big problems in the dairy industry by affecting milk quality and production. The imprudent use of antibiotics by farmers led to the appearance of resistant bacterial strains. The objective of this study was to isolate, identify Staphylococcus aureus from different farms in Khartoum State and to study its antibiotic sensitivity pattern. A total of 186 milk samples were collected from three areas in Khartoum State. After isolation and identification using Manitol salt agar media and biochemical test, 25.3 % of the samples were tested positive for Staphylococcus aureus. The identification of the isolated bacteria was also confirmed using PCR technique targeting nuc gene. Regarding sensitivity tests, the bacteria was found to be resistant to Penicillin while susceptible to Sulphamethoxazole/Trimethoprim and Ciprofloxacine.