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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 592-600
DOI: https://doi.org/10.20546/ijcmas.2018.707.072


Isolation, Characterization and Sensitivity Pattern of Staphylococcus aureus Isolated from Milk Samples in Khartoum State–Sudan
Alla Eldein Ibrahim1, Hind A. Elnasri2*, Mona A.M. Khaeirand Adil M.A. Salman1
1Department of Preventive Medicine
2Department of Biochemistry and Molecular Biology
3Department of Parasitology, College of Veterinary Medicine, University of Bahri, Sudan
*Corresponding author
Abstract:

Milk is considered as an important food material for consumers of all age groups. Albeit that, it can harbor a number of pathogens including Staphylococcus aureus which causes a big problems in the dairy industry by affecting milk quality and production. The imprudent use of antibiotics by farmers led to the appearance of resistant bacterial strains. The objective of this study was to isolate, identify Staphylococcus aureus from different farms in Khartoum State and to study its antibiotic sensitivity pattern. A total of 186 milk samples were collected from three areas in Khartoum State. After isolation and identification using Manitol salt agar media and biochemical test, 25.3 % of the samples were tested positive for Staphylococcus aureus. The identification of the isolated bacteria was also confirmed using PCR technique targeting nuc gene. Regarding sensitivity tests, the bacteria was found to be resistant to Penicillin while susceptible to Sulphamethoxazole/Trimethoprim and Ciprofloxacine.


Keywords: Antibiotic sensitivity, Milk, Staphylococcus aureus, Sudan
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How to cite this article:

Alla Eldein Ibrahim, Hind A. Elnasri, Mona A.M. Khaeir and Adil M.A. Salman. 2018. Isolation, Characterization and Sensitivity Pattern of Staphylococcus aureus Isolated from Milk Samples in Khartoum State–Sudan.Int.J.Curr.Microbiol.App.Sci. 7(7): 592-600. doi: https://doi.org/10.20546/ijcmas.2018.707.072