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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 551-564
DOI: https://doi.org/10.20546/ijcmas.2018.707.068


Phenotypic and Genotypic Characterization of Antibiotic Resistant Staphylococcus aureus in Bovine Milk Samples in Ludhiana, Punjab, India
Maliha Gulzar, Randhir Singh, Simranpreet Kaur and J.P.S. Gill
School of Public Health and Zoonoses, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhaina-141004, Punjab, India
*Corresponding author
Abstract:

A brief study was carried on fourty Staphylococcus aureus isolates from Bovine milk from Ludhiana, Punjab, India for detecting the antibiotic resistance profile using E-strips and genotypically targeting various antibiotic resistance genes. The antibiotics used for phenotypic resistance detection were Penicillin, Gentamicin, Erythromycin, Clindamycin, Tetracycline, Chloramphenicol, Ciprofloxacin, Trimethoprim-sulphamethoxazole, Oxacillin, Vancomycin andAmoxyclav. The resistance genes targeted were blaZ (Penicillin); ermA, ermB, ermC (Erythromycin); tetK, tetL, tet M, tetO (Tetracycline); aacA-aphD (Gentamycin), mecA (Oxacillin). This study provides a bird view of antimicrobial susceptibility pattern of Staphylococcus aureus in Ludhiana and can help to form scientific basis for field Veterinarians to make therapy decisions empirically thus forming a pillar in combating the antibiotic resistance problem. This study also reiterates the usefulness of PCR based technique for detection of antibiotic resistance accompanying the phenotypic tests.


Keywords: Antibiotic resistance, E-test, Multiplex PCR
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How to cite this article:

Maliha Gulzar, Randhir Singh, Simranpreet Kaur and Gill, J.P.S. 2018. Phenotypic and Genotypic Characterization of Antibiotic Resistant Staphylococcus aureus in Bovine Milk Samples in Ludhiana, Punjab, India.Int.J.Curr.Microbiol.App.Sci. 7(7): 551-564. doi: https://doi.org/10.20546/ijcmas.2018.707.068