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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 490-499
DOI: https://doi.org/10.20546/ijcmas.2018.707.060


Sequence Characterization of Ovine MHC Class II (DRB I Gene) in Indian Sheep Breeds
Kush Shrivastava1, Pushpendra Kumar1*, Mohd. Faheem Khan1, Shankar Dayal1, Nihar Ranjan Sahoo1, Rebeka Sinha1, Om Prakash1, Amit Kumar1, Manjit Panigrahi1, Anuj Chauhan1, Bharat Bhushan1, Arvind Prasad2, A. Nasir2 and B.H.M. Patel3
1Division of Animal Genetics
2Division of Parasitology, ICAR – Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., India
3LPM Section, ICAR – Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P.
*Corresponding author
Abstract:

The major histocomtability complex in sheep is also called as Ovar- MHC and is a central molecule for antigen presentation coding for specialized antigen presenting glycoproteins. Studies related to internal parasitic infestations and their association with genetic diversity in MHC region has suggested that some alleles of MHC DRB gene might augment the immune response against parasitic antigens and also may hamper parasitic fecundity inside the host. The exon 2 of DRB 1 gene forms the part of antigen loading clamp of MHC and hence carries the greatest importance in relation to disease resistant research. We sequenced the target exon in four Indian sheep breeds, the sequences were submitted to NCBI. The sequences were then studied using bioinformatic tools to ascertain its structural features and phylogenetic relationship. Finally it was observed that the variation between the sequences is majorly present in intronic region which forms an excellent marker to study the inheritance of adjoining exon also. Variations within the exons were mostly neutral and didn’t reflect in secondary structure as well as there was no change in the ligand binding site.


Keywords: Ovine MHC DRB, Exon 2, Bioinformatics, Disease resistance, Sheep
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How to cite this article:

Kush Shrivastava, Pushpendra Kumar, Mohd. Faheem Khan, Shankar Dayal, Nihar Ranjan Sahoo, Rebeka Sinha, Om Prakash, Amit Kumar, Manjit Panigrahi, Anuj Chauhan, Bharat Bhushan, Arvind Prasad, A. Nasir and Patel, B.H.M. 2018. Sequence Characterization of Ovine MHC Class II (DRB I Gene) in Indian Sheep Breeds.Int.J.Curr.Microbiol.App.Sci. 7(7): 490-499. doi: https://doi.org/10.20546/ijcmas.2018.707.060