National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Phytoplasma disease symptoms were observed on chickpea growing fields in Kurnool district, Andhra Pradesh, India. The symptoms included phyllody, pale green leaves, bushy appearance and excessive axillary proliferation. The causal agent of the phyllody disease was identified based on symptoms, amplification of 16S rDNA of the phytoplasma by nested PCR with primers P1/P7 and R16F2n/R16R2 and 1,800 bp and 1,200 bp size products were amplified in first round PCR and nested-PCR respectively. The PCR product was cloned, sequenced and compared with the reference phytoplasma sequences collected from the database (NCBI). 16S rDNA sequences of Andhra Pradesh chickpea phyllody phytoplasma shared the highest nucleotide identity i.e., 98% with Sesame phyllody phytoplasma 16SrII-D (KP297862), this is the first report of a ‘Candidatus Phytoplasma aurantifolia’ of the 16SrII-D group infecting chickpea from Andhra Pradesh.
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