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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 171-176
DOI: https://doi.org/10.20546/ijcmas.2018.707.021


Natural Occurrence of Phytoplasma Associated with Chickpea Phyllody in Andhra Pradesh
D. Vijay Kumar Naik1, B.V. Bhaskara Reddy2*, J. Sailaja Rani3, R. Sarada Jayalakshmi Devi1 and K.V. Hari Prasad4
1Department of Plant Pathology
4Department of Entomology,S. V. Agricultural College, Tirupati, India
2Department of Plant Pathology, IFT, Regional Agricultural Research Station, Tirupati, India
3Department of Plant pathology, Agricultural College, Mahanandi, India
*Corresponding author
Abstract:

Phytoplasma disease symptoms were observed on chickpea growing fields in Kurnool district, Andhra Pradesh, India. The symptoms included phyllody, pale green leaves, bushy appearance and excessive axillary proliferation. The causal agent of the phyllody disease was identified based on symptoms, amplification of 16S rDNA of the phytoplasma by nested PCR with primers P1/P7 and R16F2n/R16R2 and 1,800 bp and 1,200 bp size products were amplified in first round PCR and nested-PCR respectively. The PCR product was cloned, sequenced and compared with the reference phytoplasma sequences collected from the database (NCBI). 16S rDNA sequences of Andhra Pradesh chickpea phyllody phytoplasma shared the highest nucleotide identity i.e., 98% with Sesame phyllody phytoplasma 16SrII-D (KP297862), this is the first report of a ‘Candidatus Phytoplasma aurantifolia’ of the 16SrII-D group infecting chickpea from Andhra Pradesh.


Keywords: 16SrDNA, Chickpea, Candidatus Phytoplasma aurantifolia and Nested PCR
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How to cite this article:

Vijay Kumar Naik, D., B.V. Bhaskara Reddy, J. Sailaja Rani, R. Sarada Jayalakshmi Devi and Hari Prasad, K.V. 2018. Natural Occurrence of Phytoplasma Associated with Chickpea Phyllody in Andhra Pradesh.Int.J.Curr.Microbiol.App.Sci. 7(7): 171-176. doi: https://doi.org/10.20546/ijcmas.2018.707.021