National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Biochemical variability among the 15 isolates of Alternaria isolates from different locations of West Bengal was investigated in respect of isozyme to observe the polymorphism among the isolates. Electrophoretic separation of the extracts was carried out on native PAGE following the procedure and gels were stained for different enzymes. The activity of α- esterase produced distinctive bands that were dark brown, whereas the activity of β- esterase produced bands that were dark pink so that α- esterase and β- esterase were scored very easily. Two separate runs were conducted to determine reproducibility of the bands and to calculate the relative mobility (Rm). Values of each of the isozyme based on the presence or absence matrix of the bands of each isolate. Positive activity was observed for both α- and β- esterase. α- esterase enzyme showed the highest enzyme activity in terms of maximum numbers of banding loci among the two isozymes tested.
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