International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:7, July, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 32-39

Identification of Biochemical Variation in Alternaria Isolates of Onion Plant
Srujani Behera1, Subrata Dutta2 and Srikanta Das2
1Department of Plant Pathology, College of Agriculture, Bhawanipatna, OUAT, Odisha 766001, India
2Department of Plant Pathology, Faculty of Agriculture, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia-741252, West Bengal, India
*Corresponding author

Biochemical variability among the 15 isolates of Alternaria isolates from different locations of West Bengal was investigated in respect of isozyme to observe the polymorphism among the isolates. Electrophoretic separation of the extracts was carried out on native PAGE following the procedure and gels were stained for different enzymes. The activity of α- esterase produced distinctive bands that were dark brown, whereas the activity of β- esterase produced bands that were dark pink so that α- esterase and β- esterase were scored very easily. Two separate runs were conducted to determine reproducibility of the bands and to calculate the relative mobility (Rm). Values of each of the isozyme based on the presence or absence matrix of the bands of each isolate. Positive activity was observed for both α- and β- esterase. α- esterase enzyme showed the highest enzyme activity in terms of maximum numbers of banding loci among the two isozymes tested.

Keywords: Onion Alternaria, Isoenzymes, α- esterase and β-esterase

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How to cite this article:

Srujani Behera, Subrata Dutta and Srikanta Das. 2018. Identification of Biochemical Variation in Alternaria Isolates of Onion Plant.Int.J.Curr.Microbiol.App.Sci. 7(7): 32-39. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.