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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 32-39
DOI: https://doi.org/10.20546/ijcmas.2018.707.004


Identification of Biochemical Variation in Alternaria Isolates of Onion Plant
Srujani Behera1, Subrata Dutta2 and Srikanta Das2
1Department of Plant Pathology, College of Agriculture, Bhawanipatna, OUAT, Odisha 766001, India
2Department of Plant Pathology, Faculty of Agriculture, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia-741252, West Bengal, India
*Corresponding author
Abstract:

Biochemical variability among the 15 isolates of Alternaria isolates from different locations of West Bengal was investigated in respect of isozyme to observe the polymorphism among the isolates. Electrophoretic separation of the extracts was carried out on native PAGE following the procedure and gels were stained for different enzymes. The activity of α- esterase produced distinctive bands that were dark brown, whereas the activity of β- esterase produced bands that were dark pink so that α- esterase and β- esterase were scored very easily. Two separate runs were conducted to determine reproducibility of the bands and to calculate the relative mobility (Rm). Values of each of the isozyme based on the presence or absence matrix of the bands of each isolate. Positive activity was observed for both α- and β- esterase. α- esterase enzyme showed the highest enzyme activity in terms of maximum numbers of banding loci among the two isozymes tested.


Keywords: Onion Alternaria, Isoenzymes, α- esterase and β-esterase
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How to cite this article:

Srujani Behera, Subrata Dutta and Srikanta Das. 2018. Identification of Biochemical Variation in Alternaria Isolates of Onion Plant.Int.J.Curr.Microbiol.App.Sci. 7(7): 32-39. doi: https://doi.org/10.20546/ijcmas.2018.707.004