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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 3863-3873
DOI: https://doi.org/10.20546/ijcmas.2018.706.455


Effects of Pueraria tuberosa Linn Hydroalcoholic Tuber Extract on Expression of Apoptosis Associated Proteins in HT – 29 Human Colon Carcinoma Cell Line
M.R. Aruna1, D.J. Mukesh Kumar2, P. Palani2, D. Senbagam3 and B. Senthilkumar1*
1Department of Biotechnology, Muthayammal College of Arts and Science, Rasipuram, TN, India
2CAS in Botany, University of Madras, Guindy Campus, Chennai, TN, India
3Department of Microbiology, Vivekananda College of Arts and Science for Women,Tiruchengode, TN, India
*Corresponding author
Abstract:

Medicinal plants have been used as an excellent source for years together as an alternative medicine for treating various disorders including anticancer, cardiovascular disorders and immunological disorders. Pueraria tuberose linn (PT) commonly known as Indian Kudzu is a folklore medicine used in Ayurvedic formulations against various disorders. The present study reports the anticancer potential of the hydroalcoholic tuber extract of PT which is rich in isoflavones. The cytotoxicity of PT tuber extract was evaluated by MTT assay. Further the apoptotic potential of the extract was confirmed by Ethidium bromide/acridine orange (EtBr/AO) staining assay. DNA fragmentation which is considered as the hallmark of apoptosis was also evaluated for the hydroalcoholic tuber extract of PT. The cellular DNA was stained and the distribution of cells in the sub-G1, G0/G1, S and G2/M phases were assessed by flow cytometry. The extract showed a 50% inhibition of cell viability against HT-29 cells at a concentration of 63.91µg/mL. HT-29 cells exhibited apoptotic cell death, increased the expression of caspases, p53 and Bax. Altogether, the results suggest that the hydroalcoholic PT tuber extract has a better anticancer activity against the tested human colon carcinoma cell line.


Keywords: Indian Kudzu, Pueraria tuberose, HT – 29, Anticancer, Apoptosis, Cell cycle
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How to cite this article:

Aruna, M.R., D.J. Mukesh Kumar, P. Palani, D. Senbagam and Senthilkumar, B. 2018. Effects of Pueraria tuberosa Linn Hydroalcoholic Tuber Extract on Expression of Apoptosis Associated Proteins in HT – 29 Human Colon Carcinoma Cell Line.Int.J.Curr.Microbiol.App.Sci. 7(6): 3863-3873. doi: https://doi.org/10.20546/ijcmas.2018.706.455