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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 3629-3642
DOI: https://doi.org/10.20546/ijcmas.2018.706.427


Studies on Marker Assisted Background Screening of Sorghum Downy Mildew Resistant Introgressed Lines (BC3F3) in Maize
K. Sumathi1*, K. N. Ganesan2 and N. Senthil3
1Centre for Plant breeding and Genetics, TNAU Coimbatore, India
2Millet Breeding Station, TNAU Coimbatore, India
3Centre for Plant Molecular Biology, TNAU Coimbatore, India
*Corresponding author
Abstract:

The present investigation was carried out at Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India. The main objective of this research is to retain the recipient parent genome outside the target gene with help of SSR markers. For this purpose,background screening was done in the selected four introgressed lines (BC3F3) viz.,UMI 79/936-C1-7-7-7-46-2 ,UMI 79/936-C1-7-7-7-80-17 ,UMI 79/936-C1-7-7-7-92-1 and UMI 79/936-C1-7-7-7-92-7 with major QTLs for resistance to sorghum downy mildew in order to estimate the recovery of the genome of the recurrent parent. A total of 51 SSR markers, which showed distinct alleles between the parents, were selected for the genome wide background analysis.The graphical genotyping of the BC3F3SDMresistant introgressedlines revealed that the complete recovery of UMI 79genome was present in chromosome 3 in UMI 79/936-C1-7-7-7-46-2 and UMI 79/936-C1-7-7-7-80-17.The complete recovery of UMI 79genome was present in chromosome 7 in UMI 79/936-C1-7-7-7-92-1and UMI 79/936-C1-7-7-7-92-7.The genotype UMI 79/936-C1-7-7-7-46-2 has revealed that the 92.45 per cent (maximum recovery) of the genome were derived from the recurrent parent UMI 79,genotype UMI 79/936-C1-7-7-7-80-17 andUMI 79/936-C1-7-7-7-92-7 showed that 89.68 per cent and 80.6 per cent ofrecovery respectively.


Keywords: Back ground screening,SSR Markers, Sorghum Downy Resistant lines, Maize
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How to cite this article:

Sumathi, K., K. N. Ganesan and Senthil, N. 2018. Studies on Marker Assisted Background Screening of Sorghum Downy Mildew Resistant Introgressed Lines (BC3F3) in Maize.Int.J.Curr.Microbiol.App.Sci. 7(6): 3629-3642. doi: https://doi.org/10.20546/ijcmas.2018.706.427