Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 3559-3565
DOI: https://doi.org/10.20546/ijcmas.2018.706.418


Analysis of Genetic Diversity of Commercial Tomato Varieties using Molecular Marker viz. RAPD
Jotshana Manik Maske*, Zote Rahul Keshavrao and Rajput Charansing Amarsing
Department of Plant Biotechnology SDMVM’s College of Agricultural Biotechnology, Georai Tanda, Paithan Road, Aurangabad (M.S.)- 431001, India
*Corresponding author
Abstract:

The present Study Analysis of genetic diversity of commercial tomato varieties using molecular marker viz. RAPD was carried out at carried out at Department of Plant Biotechnology SDMVM’s College of Agricultural Biotechnology, Georai Tanda, Paithan Road, Aurangabad (M.S.), 431001 with an objectives 1.Extraction of DNA from commercial tomato varieties. 2.To analyzed genetic diversity of commercial tomato variety using molecular marker viz. RAPD. The information about the genetic diversity will be very useful for proper identification and selection of appropriate parents for breeding programmed including gene mapping, and ultimately for emphasizing the importance of marker assisted selection (MAS) in tomato improvement worldwide. PCR based molecular marker RAPD allows the rapid detection of DNA polymorphisms from many individuals samples in order to avoid erratic amplifications, good quality of genomic DNA free from contaminations and standardization of PCR reaction concentration is a perquisite for developing strategies for crop improvement programs in future. Out of those two primers, both of shows polymorphisms. In the result the OPA-11 primer shows 25% polymorphisms and the OPG-19 primer shows 66.66% polymorphisms.


Keywords: Genetic diversity, Tomato, Molecular marker, RAPD
Download this article as Download

How to cite this article:

Jotshana Manik Maske, Zote Rahul Keshavrao and Rajput Charansing Amarsing. 2018. Analysis of Genetic Diversity of Commercial Tomato Varieties using Molecular Marker viz. RAPD.Int.J.Curr.Microbiol.App.Sci. 7(6): 3559-3565. doi: https://doi.org/10.20546/ijcmas.2018.706.418