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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 3551-3558
DOI: https://doi.org/10.20546/ijcmas.2018.706.417


Evaluation of Genetic Fidelity among Two Different Cultivars of Gerbera (Gerbera jamesonii Bolus) Using DNA Based Molecular Marker RAPD
Jotshana Manik Maske*, Rajput Charansing Amarsing and Zote Rahul Keshavrao
Department of Plant BiotechnologySDMVM’s College of Agricultural Biotechnology, GeoraiTanda, Paithan Road, Aurangabad (M.S.)- 431001, India
*Corresponding author
Abstract:

The present Study entitled “RAPD Based Evaluation of Genetic Fidelity of in vitro Propagated Gerbera (Gerbera jamesonii Bolus) Variety 1385 and 1201” was carried out at carried out at Department of Plant Biotechnology SDMVM’s College of Agricultural Biotechnology, Georai Tanda, Paithan Road, Aurangabad (M.S.), 431001. The present study has been planned to evaluate genetic fidelity among two different cultivars of gerbera using DNA based molecular marker RAPD with following objectives, DNA extraction from Gerbera varietes 1385 and 1201 and Evaluation of Genetic Fidelity in vitro Propagated Gerbera (Gerbera jamesonii Bolus) from Variety 1385 and 1201.The result of the present study clearly indicated that the varieties viz-1385 & 1201 have shown monomorphic banding pattern with the primer OPA 19. The plantlet of both the varieties were also tested to see the genetic variation and it has been observed that the plantlet have also shown monomorphic banding with primer OPA19, Clearly indicating their homozygocity.


Keywords: Genetic fidelity, Gerbera, DNA, RAPD
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How to cite this article:

Jotshana Manik Maske, Rajput Charansing Amarsing and Zote Rahul Keshavrao. 2018. Evaluation of Genetic Fidelity among Two Different Cultivars of Gerbera (Gerbera jamesonii Bolus) Using DNA Based Molecular Marker RAPD.Int.J.Curr.Microbiol.App.Sci. 7(6): 3551-3558. doi: https://doi.org/10.20546/ijcmas.2018.706.417