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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 3464-3472
DOI: https://doi.org/10.20546/ijcmas.2018.706.406


Water Absorption and the Genetic Relationship of Four Different Types of Acha (Digitaria exilis) Grains Using PCR
M. F. Istifanus1*, E. B. Agbo2 and A. F. Umar2
1Department of Microbiology, Plateau State University, Bokkos, Plateau State Nigeria
2Department of Biological sciences, Abubakar Tafawa-Balewa University Bauchi, Bauchi State, Nigeria
*Corresponding author
Abstract:

The genomic DNA extraction of four different Acha types based on colour (yellow, brown, cream and white) was carried out using five (5) set of microsatellite primers. The PCR result revealed the genetic relationship and difference between the Acha types, where two of the primers did not amplify the grain, whereas three did. The water absorption behaviors of the grains were also determined using two water temperatures 35oc and 500c. There was an initial rapid rate of water absorption within the first hour which later reduces and become steady from 6 hours up to the 24thhours of steeping. The summary of the analysis reveals that yellow, brown and white colour types are not significantly different at p-value confidence level.


Keywords: Extraction, water absorption steeping
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How to cite this article:

Istifanus M. F., E. B. Agbo and Umar A. F. 2018. Water Absorption and the Genetic Relationship of Four Different Types of Acha (Digitaria exilis) Grains Using PCR.Int.J.Curr.Microbiol.App.Sci. 7(6): 3464-3472. doi: https://doi.org/10.20546/ijcmas.2018.706.406