International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:6, June, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 2749-2756

Isolation and Characterization of Phosphate Solublizing Bacteria from Rhizosphere Soil of Rice in Jammu District, India
Renu Gupta1*, Harkirat Singh1, Bharti Sharma2 and Susheel Verma2
1Division of Soil Science and Agricultural Chemistry, Faculty of Agriculture, SKUAST-J, Chatha, Jammu, India
2Centre for Biodiversity Studies, School of Biosciences and Biotechnology, BGSB University, Rajouri-185234, J&K, India
*Corresponding author

Phosphorous is one of the important macronutrient along with nitrogen which limit the growth, development, and yield of the plant. The soluble forms of phosphorus when applied to soil become insoluble through chemical fixation. The Phosphate solublizing bacteria (PSB) plays a very vital role in making soluble phosphorous available to the plants. A study was undertaken in rice growing area of Jammu district, J&K, to screen the phosphate solubilizing microorganisms from the rhizosphere soil. The soil samples were collected from two rice growing blocks, R. S. Pura and Bishnah using Global Positioning System. The study revealed the presence of three dominant genera. They were identified following Gram staining method and some biochemical tests. Their phosphate solubilizing ability was screened by halozone test on solid agar plate after the incubation at 28 °C. The observations were recorded at 2nd,4th and 6th day interval. The microbes showed the formation of clear zones around their colonies on agar plate. The dominant genera were identified as Bacillus, Pseudomonas and Enterobacter on the basis of biochemical test performed on various isolates these were subjected to identification and placed in group I,VIII and IX by referring to the separation outlined in Bergeys manual of systematic bacteriology Thus, it was observed that PSB from the three genera have the ability to reduce the phosphate unavailability by making it available to plants and can be used as biofertilizers in soil because these microorganisms neutralize the soil and release acid in minute quantity during phosphate solubilization.

Keywords: Phosphorus, Phosphate-Solubilizing Bacteria (PSB), Isolation, Biochemical characterization, identification

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How to cite this article:

Renu Gupta, Harkirat Singh, Bharti Sharma and Susheel Verma. 2018. Isolation and Characterization of Phosphate Solublizing Bacteria from Rhizosphere Soil of Rice in Jammu District, India.Int.J.Curr.Microbiol.App.Sci. 7(6): 2749-2756. doi:
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