National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Four Swamp buffalo bulls aged 5 to 8 years were used to study the fresh semen as well as biochemical characteristics. A total of 60 ejaculates comprising 15 from each bull evaluated for fresh semen characteristics and a total of 40 ejaculates comprising 10 from each bull were used for evaluation and biochemical characterization. For Biochemical constituents of semen the AST and ALT activities, total protein, total cholesterol and total lipid concentration were estimated in seminal plasma of fresh semen and the overall mean values of the AST and ALT activities and total protein, total cholesterol and total lipid concentration were 26.05±0.74 U/L, 49.14±1.72 U/L, 3.44±0.02 mg/ml, 23.12±0.65 mg/dl and 2.20±0.07 mg/ml respectively in seminal plasma of fresh semen. The sperm membrane extract and seminal plasma proteins of fresh semen characterized by SDS-PAGE. Seven protein bands in seminal plasma and eight protein bands were identified in sperm membrane extract of the Swamp buffalo semen.
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